Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease

Table 2

Proliferative responses to  𝛼- and 𝛾-gliadin epitopes.

Epitope	Amino acid sequence*	Proliferation response**
Native 𝛼-gliadin	SGQGSFQPSQQNP	1/10 (10%)
𝛼-Gliadin p1E	SGE GSFQPSQQNP	6/10 (60%)
𝛼-Gliadin p9E	SGQGSFQPSQ ENP	2/10 (20%)
𝛼-Gliadin p1E, p9E	SGE GSFQPSQ ENP	0/10 (0%)
𝛼-I-Gliadin	QLQPFPQP E LPY	3/10 (30%)
𝛼-II-Gliadin	PQP E LPYPQPQL	0/10 (0%)
Native 𝛾-gliadin	FPQQPQQPYPQQPQQ	0/10 (0%)
𝛾-Gliadin p1E	FPE QPQQPYPQQPQQ	1/10 (10%)
𝛾-Gliadin p9E	FPQQPQQPYP EQPQQ	0/10 (0%)
𝛾-Gliadin p1E, p9E	FPE QPQQPYP EQPQQ	2/10 (20%)
𝛾-I-Gliadin	PEQPQQSFP E QERP	0/10 (0%)

∗Glutamic acid (E) residues in bold are formed by tissue transglutaminase mediated deamidation. Underlined residues form the MHC class II peptide binding register.
^∗∗A stimulation index ≥ 3 is considered a response.