Differential Immunotoxicity Induced by Two Different Windows of Developmental Trichloroethylene Exposure

Table 1

Primer sequences used for qRT-PCR.

Gene		Primer sequences 5′ to 3′	Tm (°C)
Aire	Sense	AGATCGCGGTGGCCATAG	57.4
	Antisense	TCGTGGTCGGCTAGAGCAT	58.4
Bax	Sense	TTGCTGATGGCAACTTCAACTGGG	60.2
	Antisense	TGTCCAGCCCATGATGGTTCTGAT	60.4
Bim	Sense	CTGTGTAATGTGCCCTACTGTTTC	55.8
	Antisense	GGAAGAGAACCAGCCACTACC	57.3
CD70	Sense	TGCTGGTGGTGTTTATTACTGTG	55.4
	Antisense	CTCTGGTCCGTGTGTGAAGG	57.7
Cdkn1a	Sense	AATCCTGGTGATGTCCGACCTGTT	60.2
	Antisense	GTGACGAAGTCAAAGTTCCACCGT	59.4
Dnmt1	Sense	TGATAAGGAGGACAAGGAGAATGC	56.4
	Antisense	CACCGCCAAGTTAGGACACC	58.3
Dnmt3a	Sense	CAGCACCATTCCTGGTCATGCAAA	60.2
	Antisense	TCCTGTGTGGTAGGCACCTGAAAT	60.2
Fas	Sense	CGCCCGCTGTTTTCCC	57.6
	Antisense	GCAAGCACCAGAGGCAGG	59.4
FasL	Sense	GGCTGGGTGCCATGCA	59.4
	Antisense	GGCACTGCTGTCTACCCAGAA	59.2
IAP	Sense	GCACCCTCAAAGCCTATCTTAT	54.6
	Antisense	TCCCTTGGTCAGTCTGGATTT	55.8
Ifng	Sense	AGCTCATCCGAGTGGTCCAC	59.1
	Antisense	AGCAGCGACTCCTTTTCCG	57.8
Il2	Sense	CCCAAGCAGGCCACAGAATTGAAA	60.2
	Antisense	AGTCAAATCCAGAACATGCCGCAG	59.9
Il4	Sense	AGCCATATCCACGGATGCGACAAA	60.8
	Antisense	AATATGCGAAGCACCTTGGAAGCC	60.0
Il6	Sense	AGAGGAGACTTCACAGAGGATACC	57.1
	Antisense	CATTTCCACGATTTCCCAGAGAAC	56.1
Muerv	Sense	TGGTGGTCGAGATGGAGGTTA	57.5
	Antisense	CCGTGAATGGTGGTTTTAGCA	55.8