Heavy Metal-Induced Oxidative DNA Damage in Earthworms: A Review
Figure 4
Immunohistochemical analyses of 8-oxo-dG accumulation in the gut epithelial layers and seminal vesicles (SV) of E. fetida (S1). Controls for the gut epithelial layer: 1 week (a), 2 weeks (c), 3 weeks (e). The gut epithelial layer of the Cd (200 g/g soil) treatment group: 1 week (b), 2 weeks (d), 3 weeks (f). (g) Negative staining of Cd (200 g/g soil, 1 week) with a 2% bovine serum albumin solution. Arrowheads show the gut epithelial layer. AC: alimentary canal. (h) The SV. Arrowheads show positive signals in SV. All scale bars are 100 m. (Data of (h) are modified by Nakashima et al. [18]).
