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Applied and Environmental Soil Science
Volume 2012 (2012), Article ID 239639, 11 pages
http://dx.doi.org/10.1155/2012/239639
Research Article

Control of Fusarium Wilt of Tomato Caused by Fusarium oxysporum F. Sp. Radicis-Lycopersici Using Mixture of Vegetable and Posidonia oceanica Compost

1Laboratory of Treatment and Water Recycling, Centre of Research and Water Technologies (CERTE), Technopark of Borj-Cedria, P 273, 8020 Soliman, Tunisia
2JICA International Japanese Cooperation Agency, Tunisia
3University of California Cooperative Extension 669 County Square Drive, Suite 100 Ventura, CA 93003-805, USA
4University of El Manar, Faculté des Sciences de Tunis, Campus, Laboratoire de Biomolécules Actives, Tunisia

Received 4 December 2011; Revised 25 March 2012; Accepted 30 March 2012

Academic Editor: Alejandro Valdecantos

Copyright © 2012 S. Kouki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A compost of vegetable waste and Posidonia oceanica mixture (70 : 30% vol : vol) was tested in vitro and in vivo for its efficacy against Fusarium oxysporum f.sp radicis-lycopersici (Forl), the causal agent of Fusarium wilt of Tomato (Lycopersicon esculentum cv. chourouk).The incorporation of non-sterilized VPC in the culture medium showed potent antifungal activity against Forl and complete inhibition of mycelium growth was observed for all the tested compost rates (0.5, 1, 2, 4, 6, 8, 10, 15 and 20%). However, only the highest rates (15 and 20%) of a sterilized suspension of VPC were effective in preventing mycelial growth. Nine indigenous bacterial strains isolated from VPC exhibited antagonism against Forl. Based on 16S rDNA sequence analysis, the isolates were assigned to Bacillus sphaericus (B12 and BS2), Pseudomonas putida PPS7 and Burkholderia gladioli BuC16. Under green house condition, seed inoculation by B12, BS2, PP7 and BuC16 strains protected significantly tomato against Fusarium oxysporum f.sp radicis-lycopersici (Forl) attacks.