- About this Journal ·
- Abstracting and Indexing ·
- Advance Access ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Applied and Environmental Soil Science
Volume 2013 (2013), Article ID 835960, 5 pages
Residual Effects of Biosolids Application on Forage Production of Semiarid Grassland in Jalisco, Mexico
1Sitio Experimental La Campana, CIRNOC, INIFAP, Km 33 Carretera Chihuahua-Ojinaga, 32910 Aldama, CHIH, Mexico
2Sitio Experimental Vaquerias, CIRPAC, INIFAP, Km 8 Carretera Ojuelos-Lagos de Moreno, 47540 Ojuelos, JAL, Mexico
3Departamento de Zootecnia, Centro de Ciencias Agropecuarias, Universidad Autonoma de Aguascalientes, Avenida Universidad No. 940, 20131 Aguascalientes, AGS, Mexico
Received 31 March 2013; Revised 26 May 2013; Accepted 14 June 2013
Academic Editor: Alejandro Valdecantos
Copyright © 2013 Pedro Jurado-Guerra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Single application of biosolids increases forage production on semiarid grasslands. Residual effects of biosolids on forage production have been scarcely measured in semiarid grasslands. The objective was to evaluate the residual effects of biosolids application on forage production of blue grama (Bouteloua gracilis (Willd. ex Kunth) Lag. ex Griffiths) and other grasses at a semiarid grassland in Jalisco, Mexico. The study was performed at shortgrass prairie in northeast Jalisco. Field plots were selected to include blue grama plants before rainy season in 2002. Aerobic biosolids were applied at 0 (control), 15, 30, 45, 60, 75, or 90 dry Mg ha−1 under a completely random design with five replications. Forage production was estimated by clipping at the end of the growing season during five years. Data analysis was performed with linear mixed model and repeated measures. Forage production was influenced by a rate × year × species interaction (). Blue grama forage production increased with increasing biosolids rates during all years, with the magnitude of this response varying among years. Forage production of other grass species slightly decreased with biosolids application. Single biosolids application had a residual effect on forage production throughout five years in semiarid grasslands.
Approximately half of the world native grasslands show moderate to strong degradation , while approximately 95% of the grasslands are overgrazed and show degradation in Mexico . Studies in Mexico indicate that most of the shortgrass prairie in Jalisco , Zacatecas, and Chihuahua  show moderate-extreme to extreme rangeland health. Oak-bunchgrass and Halophyte grasslands also show deterioration, including high invasion of native shrubs and exotic grasses in Chihuahua .
Practices to recover degraded grasslands may include use of fertilizers, prescribed fire, shrub management, and soil and water conservation techniques. Fertilizer application to native grasslands has increased forage production [6, 7] although high fertilizer costs make this a low profitability technique. An alternative might be application of organic byproducts such as biosolids that are generated at wastewater treatment plants and can be used as fertilizer and soil amendments [8, 9].
Several studies have shown that surface-applied biosolids promote grass production at arid and semiarid grasslands [10–12]. Biosolids rates from 18 to 45 Mg ha−1 have been recommended for arid rangelands with good agronomic results in terms of grass forage production and soil fertility . Also, residual effects have been observed on soil fertility [14–16] and on forage production of arid grasslands [10, 11]. However, limited information is available on the residual effects of biosolids application in semiarid grasslands. Then, the objective of this study was to evaluate the residual effects of biosolids application on forage production of blue grama and other grasses at a semiarid grassland in Jalisco, Mexico.
2. Materials and Methods
The study was conducted at the Vaquerias Experimental Station, INIFAP, located at km 8 of Ojuelos-Lagos de Moreno highway, Ojuelos, Jalisco, Mexico, at 21.77927°N, 101.61133°W at 2,150 m altitude. Climate is semitropical  with a mean annual temperature at 17°C and mean annual precipitation at 425 mm. Topography is flat to less than 3% slope. Soil is Haplic Calcisol , alluvial origin, sandy loam texture, and a cement phase (“tepetate”) with a 50–100 cm soil depth. Dominant vegetation is composed of Bouteloua-Lycurus with huizache (Acacia schaffneri (S. Watson) F.J. Herm.) and prickly pear (Opuntia spp.) invasion .
Thirty-five field plots 1 × 1 m were selected at a native shortgrass prairie at regular range condition including blue grama (Bouteloua gracilis (Willd. ex Kunth) Lag. ex Griffiths) plants with an initial mean forage production of 843 ± 101 kg ha−1 estimated by clipping before biosolids application. Square plots with a plywood frame were used to retain biosolids. Domestic, aerobic biosolids from the wastewater treatment plant at Aguascalientes city, Mexico, were applied one time only at 0 (control), 15, 30, 45, 60, 75, or 90 Mg ha−1 on a dry weight basis, with five replications in June 2002. Previous to biosolids application, four random 1 kg samples were taken to determine moisture content and the following chemical analysis (Table 1): pH (CaCl2/electrometric pH meter); electrical conductivity (saturated paste/Solu-bridge); organic matter through Walkley and Black combustion method ; total nitrogen (Kjeldahl) ; phosphorus (Bray-1) ; potassium, calcium, and magnesium, extracted by ammonium acetate; aluminum, zinc, iron, copper, manganese, boron, lead, arsenic, chromium, and cadmium extracted by DTPA  and analyzed by Atomic Absorption Spectroscopy; sulfur was extracted with calcium chloride and determined by turbidimetric method and chloride by volumetric methods. Chemical analysis of biosolids (Table 1) showed that pH and electrical conductivity were lower, and organic matter, nitrogen, and phosphorus were slightly higher than values in other studies [10, 11]. Because of their N and P contents, biosolids have the potential to improve soil fertility and promote plant growth at different agricultural scenarios. Biosolids composition complies with the Official Regulation of SEMARNAT in Mexico and was rated “Excellent” in terms of metal content .
Chemical analyses of initial soil samples before biosolids application were done similar to biosolids and are shown as follows (mean ± SE): pH = 6.3 ± 0.03; electrical conductivity = 0.028 ± 0.001 dS m−1; organic matter = 1.28 ± 0.03%; extractable nitrogen = 3 ± 0.3 mg kg−1; extractable mg kg−1; and extractable potassium = 352 ± 10 mg kg−1.
Biosolids rates were randomly assigned to field plots . Biosolids were surface-applied and uniformly distributed around the plots by hand at the beginning of the rainy season in June 2002. Plots were excluded to grazing during the study and kept under natural rainfall conditions. Precipitation was measured at a meteorological station close to the study site (Table 2). Forage production was estimated by clipping blue grama forage separated from other grasses including hairy grama (Bouteloua hirsuta Lag.), poverty three-awn (Aristida divaricata Humb. & Bonpl. Ex Willd.), common wolfstail (Lycurus phleoides Kunth), or Bouteloua scorpioides Lag. at 5 cm stubble height at the end of the growing season each year during four years. Forage production was determined by oven-drying forage samples at 60°C during 48 hr and is expressed on a dry weight basis.
Forage production data was analyzed with linear mixed models and year as repeated measures . The first analysis included forage production of blue grama and other grasses from 2003 to 2004 to estimate changes of blue grama and other grasses production over time. The second analysis included total forage production (sum of blue grama and other grasses) from 2003 to 2006 to determine residual effect of biosolids on forage production. Several covariance structures including unstructured variance, compound symmetry, heterogeneous compound symmetry, and autoregressive were tested to select the best models. The models with unstructured variance were selected due to lower Akaike Information Criterion . Significant effects were declared at a 5% probability level.
3. Results and Discussion
The analysis on forage production of blue grama and other grasses was affected () by a rate × year × species interaction (Table 3). Blue grama and other grasses forage production varied as a response to biosolids application and year of forage production (Figure 1). Blue grama forage increased from 2,533 ± 354 to 7,224 ± 354 kg ha−1 in 2003 and from 2,375 ± 227 to 5,385 ± 227 kg ha−1 in 2004, with 15 and 90 Mg ha−1 biosolids rates, respectively (Figure 1) showing a high residual effect. In contrast, forage production of other grasses was low and decreased from 106 to 30 kg ha−1 in 2003 and from 181 to 36 kg ha−1 in 2004 at the same rates (Figure 1). At control rate, forage production of blue grama (mean ± SE; 475 ± 148 kg ha−1) was similar () compared to other grasses (488 ± 148 kg ha−1) in 2003 and slightly higher () in 2004 (Figure 1).
Similar trends on increasing forage production of blue grama with biosolids and slighter effects of biosolids on other grasses such as galleta (Hilaria jamesii (Torr.) Benth.) and bottlebrush squirreltail (Sitanion hystrix (Nutt.) J.G. Sm.) have also been observed on arid grasslands in New Mexico . However, blue grama forage production was lower in their study varying from 392 kg ha−1 at control rate to a maximum of 1,067 kg ha−1 at a biosolids rate of 90 Mg ha−1, probably attributed to a lower mean annual precipitation of 267 mm in their study site. Blue grama forage production was much higher in our study due to more favorable growing conditions such as a higher precipitation in the study site (Table 2). Another study showed variable effects of composted biosolids on plant cover in a shortgrass prairie dominated by western wheatgrass (Pascopyrum smithii (Rydb.) Love) and needle and thread grass (Hesperostipa comata (Trin & Rupr.) Barkworth) in a semiarid grassland in Colorado with 355 mm annual rainfall .
The residual effect of biosolids application on total forage production was affected () by a rate × year interaction (Table 3). Total forage production increased with biosolids application rates, and the effect was greater in 2003 and 2004 (Figure 2), showing a residual effect even four years after biosolids application. Increases of forage production varied from 5 to a maximum of 15 times at 15 to 90 Mg ha−1 rates, respectively, in 2003. However, the residual effect was lower in 2005 and 2006 attributed to a low precipitation during the growing season in both years (Table 2). Results of a similar study in New Mexico  showed increases of forage production up to 74% at the second year and up to 74% at the fourth year with 45 Mg ha−1 rate at an arid blue grama grassland in poor range condition. Research in Texas  showed a maximum increase of 92% on forage production of tobosa grass (Pleuraphis mutica Buckley) at the fourth year with single application of 36 Mg ha−1 rate and up to 80% with double annual applications of biosolids at 35 Mg ha−1 rate in arid grasslands . The lower forage production in New Mexico and Texas can be attributed to low precipitation in their study sites with 267 and 310 mm of mean annual precipitation and lower than average at three out of four years during the study.
Also, forage production of winter grasses has increased with biosolids application at 40 to 120 Mg ha−1 rates after three years of application at a degraded native grassland in Spain . A later study  showed that forage production only increased at 80 and 120 Mg ha−1 rate after five years of application at the same site.
Our data showed the greatest forage production of grasses at native grasslands obtained under rainfed conditions, and they could be partially attributed to the precipitation observed during the years of study. Besides precipitation, the higher forage production with biosolids application could also be attributed to the plant growth nutrients provided by biosolids as shown by several studies [16, 27] and to the improvement on soil water infiltration and water availability with biosolids application [16, 30].
Soil fertility was evaluated during the first and second years after biosolids application in this study , showing beneficial effects on soil nutrients such as nitrogen and phosphorus. These beneficial results on soil fertility are partially responsible for the increases on grass forage production observed in this study. Improvement on soil fertility was more beneficial to blue grama, a dominant species in this grassland that had a better use of soil nutrients; meanwhile, other associated species were displaced as biosolid rates increased.
Residual effects of biosolids on soil fertility have been observed with increases on soil nitrogen and phosphorus after eight years of application of 45 and 90 Mg ha−1 rates on degraded native grassland of blue grama-galleta (Hilaria jamesii (Torr.) Benth.) in New Mexico . Other authors also found residual effects on soil nitrogen and available phosphorus after five years with biosolids applications from 40 to 120 Mg ha−1 rates compared to control rate in native grassland in Spain . Residual effects on soil available nitrogen and phosphorus after 12 years have also been observed in a shortgrass prairie with biosolids application from 5 to 30 Mg ha−1 rates in Colorado . However, organic matter from biosolids decreased with time, and soil nitrogen availability has been shown to decrease from 5 to 1% in a seven-year period in desert grasslands in Texas .
Although biosolids application has shown favorable effects on forage production and consequently could have an impact on animal production, there are some limitations that make this practice not suitable for all grasslands. First, even though biosolids generation will increase due to increasing world population and to increasing environmental concerns for wastewater treatment, biosolids availability is still low in some remote and rural areas; second, high transportation and application costs [33, 34] make this practice more suitable for those grasslands closer to the wastewater treatment plants; and third, caution is advisable due to content of pathogens and heavy metals on biosolids. Despite this, land application of biosolids is still a recommended option to recycle organic matter and nutrients for plant growth in grasslands  and to restore degraded ecosystems .
Single biosolids application on the surface showed a residual effect on forage production even five years after application. The major effects on forage production were on blue grama at all biosolids rates. Other grasses such as hairy grama (Bouteloua hirsuta Lag.), poverty three-awn (Aristida divaricata Humb. & Bonpl. Ex Willd.), common wolfstail (Lycurus phleoides Kunth), and Bouteloua scorpioides Lag. showed no response to biosolids application. The residual effect on forage production is still very strong at 45 to 90 Mg ha−1 rates and is fading on biosolids rates from 15 to 30 Mg ha−1. A biosolids rate of 45 Mg ha−1 is recommended, based on the residual effect on forage production during the years evaluated.
Thanks are due to CONACYT for the financial support to the research Project I37450-B, to personnel at the wastewater treatment plant of Aguascalientes, Mexico, for providing biosolids, and to Rodolfo Barretero for field assistance.
- R. B. White, S. Murray, and M. Roheder, Pilot Analysis of Global Ecosystems-Grassland Ecosystems, World Resources Institute, Washington, DC, USA, 2000, http://pdf.wri.org/page_grasslands.pdf.
- SEMARNAT, Secretaría del Medio Ambiente y Recursos Naturales, Informe de la situación del medio ambiente en Mexico, Vegetacion y uso del suelo, 2005, http://www.semarnat.gob.mx/informacionambiental/.
- P. Jurado, M. Luna, and M. Royo, “Estimating rangeland health in the Highlands of Jalisco,” in Proceedings of the Monitoring Science and Technology Symposium:Unifying knowledge for sustainability in the western hemisphere, C. Aguirre-Bravo, et al., Ed., Rocky Mountain Experimental Station-USDA Forest Service, Denver, CO, USA, September 2004.
- M. Royo, A. Melgoza, J. S. Sierra et al., “La salud de los pastizales medianos en los estados de Chihuahua y Zacatecas,” in Proceedings of the II Simposio Internacional de Manejo de Pastizales, Zacatecas, Mexico, 2005.
- A. Valerio, E. Carreon, A. Lafon et al., “Extension Espacial y Condicion de los Pastizales en el Estado de Chihuahua,” in Proteccion de la Fauna Mexicana, A.C. The Nature Conservancy, Chihuahua, Mexico, 2005.
- J. T. Arredondo, “Efecto de la fertilización de nitrógeno y fósforo en un pastizal mediano abierto en el norte de Jalisco,” Tecnica Pecuaria en Mexico, vol. 47, pp. 49–59, 1984.
- H. O. Rubio, M. K. Wood, A. Gomez, and G. Reyes, “Native forage quality, quantity, and profitability as affected by fertilization in northern Mexico,” Journal of Range Management, vol. 49, no. 4, pp. 315–319, 1996.
- USEPA, United States Environmental Protection Agency, “Biosolids Technology Fact Sheet: Land Application of Biosolids,” EPA 832-F-00-064, United States Environmental Protection Agency, 2000.
- The National Research Council, Biosolids Applied To Land: Advancing Standards and Practices, National Academy Press, Washington, DC, USA, 2002.
- M. W. Benton and D. B. Wester, “Biosolids effects on tobosagrass and alkali sacaton in a Chihuahuan desert grassland,” Journal of Environmental Quality, vol. 27, no. 1, pp. 199–208, 1998.
- P. Jurado and D. B. Wester, “Effects of biosolids on tobosagrass growth in the Chihuahuan desert,” Journal of Range Management, vol. 54, no. 1, pp. 89–95, 2001.
- P. Jurado, M. Luna, R. Barretero, M. Royo, and A. Melgoza, “Produccion y calidad de forraje y semilla de zacate navajita con la aplicacion de biosolidos en un pastizal semiarido de Jalisco,” Tecnica Pecuaria en Mexico, vol. 44, pp. 289–300, 2006.
- D. B. Wester, R. E. Sosebee, R. E. Zartman et al., “Biosolids effects in Chihuahuan desert rangelands: a Ten-Year Study,” Applied and Environmental Soil Science, vol. 2011, Article ID 717863, 13 pages, 2011.
- C. S. White, S. R. Loftin, and R. Aguilar, “Application of biosolids to degraded semiarid rangeland: nine-year responses,” Journal of Environmental Quality, vol. 26, no. 6, pp. 1663–1671, 1997.
- I. Walter, F. Martínez, and G. Cuevas, “Biosolid amendment of a calcareous, degraded soil in a semi-arid environment,” Spanish Journal of Agricultural Research, vol. 4, no. 1, pp. 47–54, 2006.
- P. Jurado, T. Arredondo, E. Flores, V. Olalde, and J. Frias, “Efecto de los biosolidos sobre la humedad y nutrimentos del suelo y produccion de forraje en pastizales semiaridos,” Terra Latinoamericana, vol. 25, pp. 211–218, 2007.
- G. G. Medina, A. Ruiz, and R. Martinez, Los Climas de México. Una Estratificación Ambiental Basada en el componente Climático, Issue 1 of Libro técnico, CIRPAC-INIFAP-SAGAR, Guadalajara, Jalisco, México, 1998.
- FAO-ISSS-ISRIC, World Reference Base for Soil Resources, 1998, http://www.fao.org/W8594E00.htm.
- COTECOCA, “Comision Tecnico Consultiva para la Determinacion de Coeficientes de Agostadero,” in Coeficientes de agostadero de la Republica Mexicana, Tomo II, pp. 165–244, Estado de Jalisco, SARH-Subsecretaria de Ganaderia, Jalisco, Mexico, 1979.
- D. W. Nelson and L. E. Sommers, “Total carbon, organic carbon and organic matter,” in Methods of Soil Analysis, Part 2, A. L. Page, R. H. Miller, and D. R. Keeney, Eds., 9. ASA-SSSA, pp. 539–579, Agronomy Monogr, Madison, WI, USA, 1982.
- J. M. Bremner and C. S. Mulvaney, “Nitrogen—total,” in Methods of Soil Analysis. Part 2, A. L. Page, R. H. Miller, and D. R. Keeney, Eds., 9. ASA-SSSA, pp. 595–624, Agronomy Monogr, Madison, WI, USA, 1982.
- S. R. Olsen and L. E. Sommers, “Phosphorus,” in Methods of Soil Analysis. Part 2, A. L. Page, R. H. Miller, and D. R. Keeney, Eds., 9. ASA-SSSA, pp. 403–430, Agronomy Monogr, Madison, WI, USA, 1982.
- W. L. Lindsay and W. A. Norvell, “Development of DTPA soil test for zinc, iron, manganese and copper,” in Soil Science Society of America Journal, vol. 42, pp. 421–428, 1978.
- SEMARNAT, “SECRETARIA DEL MEDIO AMBIENTE Y RECURSOS NATURALES Normas Oficiales Mexicanas Vigentes,” NOM-004-SEMARNAT-2002-Proteccion Ambiental Lodos y Biosolidos, 2003, http://www.semarnat.gob.mx/leyesynormas/pages/resultados.aspx.
- R. G. D. Steel and J. H. Torrie, Principles and Procedures of Statistics, McGraw-Hill Book Company, New York, NY, USA, 2nd edition, 1980.
- SAS, Statistical Analysis System. Versión 9. 0., SAS Institute, Cary, NC, USA, 2008.
- P. R. Fresquez, R. E. Francis, and G. L. Dennis, “Soil and vegetation responses to sewage sludge on a degraded semiarid broom snakeweed/blue grama plant community,” Journal of Range Management, vol. 43, no. 4, pp. 325–331, 1990.
- J. A. Ippolito, K. A. Barbarick, M. W. Paschke, and R. B. Brobst, “Infrequent composted biosolids applications affect semi-arid grassland soils and vegetation,” Journal of Environmental Management, vol. 91, no. 5, pp. 1123–1130, 2010.
- F. Martínez, G. Cuevas, R. Calvo, and I. Walter, “Biowaste effects on soil and native plants in a semiarid ecosystem,” Journal of Environmental Quality, vol. 32, no. 2, pp. 472–479, 2003.
- C. A. Moffet, R. E. Zartman, D. B. Wester, and R. E. Sosebee, “Surface biosolids application: effects on infiltration, erosion, and soil organic carbon in Chihuahuan Desert grasslands and shrublands,” Journal of Environmental Quality, vol. 34, no. 1, pp. 299–311, 2005.
- T. S. Sullivan, M. E. Stromberger, M. W. Paschke, and J. A. Ippolito, “Long-term impacts of infrequent biosolids applications on chemical and microbial properties of a semi-arid rangeland soil,” Biology and Fertility of Soils, vol. 42, no. 3, pp. 258–266, 2006.
- W. F. Jaynes, R. E. Zartman, R. E. Sosebee, and D. B. Wester, “Biosolids decomposition after surface applications in west Texas,” Journal of Environmental Quality, vol. 32, no. 5, pp. 1773–1781, 2003.
- CONAGUA, Comision Nacional del Agua, Manual de agua potable, alcantarillado y saneamiento-Guia para el manejo, tratamiento y disposición de lodos residuales de plantas de tratamiento municipales, 2007, http://www.cna.gob.mx/.
- H. R. Uribe, N. Chavez, G. Orozco, and S. Espino, “Biosolidos digeridos anaerobicamente en la producción de maiz forrajero,” Agricultura Tecnica en Mexico, vol. 29, pp. 25–34, 2003.
- Q. Lu, Z. L. He, and P. J. Stoffella, “Land application of biosolids in the USA: a review,” Applied and Environmental Soil Science, vol. 2012, Article ID 201462, 11 pages, 2012.