- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Article Processing Charges ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Recently Accepted Articles ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Advances in Hematology
Volume 2011 (2011), Article ID 510304, 7 pages
Hepcidin: A Critical Regulator of Iron Metabolism during Hypoxia
1Department of Nutrition, Dietetics & Food Sciences, Utah State University, Logan, UT 84322, USA
2Military Nutrition Division, US Army Research Institute of Environmental Medicine (USARIEM), Natick, MA 01760, USA
Received 27 May 2011; Accepted 8 July 2011
Academic Editor: Angela Panoskaltsis-Mortari
Copyright © 2011 Korry J. Hintze and James P. McClung. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Iron status affects cognitive and physical performance in humans. Recent evidence indicates that iron balance is a tightly regulated process affected by a series of factors other than diet, to include hypoxia. Hypoxia has profound effects on iron absorption and results in increased iron acquisition and erythropoiesis when humans move from sea level to altitude. The effects of hypoxia on iron balance have been attributed to hepcidin, a central regulator of iron homeostasis. This paper will focus on the molecular mechanisms by which hypoxia affects hepcidin expression, to include a review of the hypoxia inducible factor (HIF)/hypoxia response element (HRE) system, as well as recent evidence indicating that localized adipose hypoxia due to obesity may affect hepcidin signaling and organismal iron metabolism.
1. Iron: An Essential Trace Element
Despite the initial identification of iron as a major component of human blood over two hundred years ago, poor iron status continues to affect billions of people worldwide. Although the prevalence of iron deficiency (diminished iron stores, ID) and iron deficiency anemia (diminished iron stores coupled with reduced hemoglobin, IDA) is greatest in the developing world, poor iron status also affects a significant portion of the population in developed nations. For example, ID and IDA affect up to 12 and 4% of premenopausal women in the United States, respectively . Iron functions biochemically through incorporation into a series of proteins and enzymes. Many of these proteins and enzymes, including myoglobin, cytochrome c, and hemoglobin, are required for optimal cognitive and physical performance in humans [2–4]. Although diminished work capacity due to reduced hemoglobin levels is the best described functional consequence of poor iron status, other outcomes include diminished intellectual performance, altered body temperature regulation, and reduced immunity and resistance to infections .
Iron balance has been historically defined as the net difference between the amount of iron that is absorbed from the diet and the amount of iron that is excreted. As such, countless investigations have focused on dietary factors affecting iron balance, such as the quantity of iron found in particular food sources and factors that may promote or inhibit iron absorption. Although iron consumption through the diet is clearly a major determinant of iron status, it has become increasingly apparent that many factors affect iron balance (Figure 1). In fact, iron balance is tightly regulated through a series of cellular transport proteins that are sensitive to the recently discovered iron regulatory hormone, hepcidin. The discovery of hepcidin, a 25-amino acid protein, has significantly altered the research paradigm in the field of iron homeostasis. Although the contribution of dietary sources to iron balance should not be ignored, hepcidin has been described as the “central regulator of iron homoeostasis,” and affects both iron absorption through the duodenal enterocyte and iron export from the macrophage and other iron exporting cells. Further, a series of factors, some of which are not directly associated with dietary iron intake, affect hepcidin levels, such as inflammation, erythropoiesis, and hypoxia. This paper will detail the mechanism by which hepcidin affects iron metabolism, with a focus on hypoxia, a factor affecting the molecular regulation of hepcidin, and, ultimately, iron status.
2. Hepcidin: The Central Regulator of Iron Homeostasis
Hepcidin was first identified as an antimicrobial peptide synthesized by the liver in 2000 [6, 7]. Although hepcidin confers antimicrobial properties in vitro, the estimated concentrations found in biological fluids may not support a major role for this peptide hormone as an antimicrobial agent in vivo . In 2001, the observation that hepcidin was overexpressed in response to dietary iron overload led to the hypothesis that hepcidin may function as a central regulator of iron homeostasis . Subsequently, roles for hepcidin in both the acquisition of iron from the gastrointestinal tract and the efflux of iron from macrophages have been characterized.
Hepcidin regulates iron homeostasis mainly through its ability to bind ferroportin 1 (FPN1), the iron exporter responsible for iron egress from duodenal enterocytes, macrophages, and other iron exporting cells. Specifically, hepcidin binds to FPN1 on the cell surface, triggering the internalization of the hepcidin-FPN1 complex, which results in the subsequent ubiquitination of the FPN1 and the degradation of the protein complex [10, 11]. Experimental evidence confirms this mechanism of hepcidin action: disruption of the hepcidin gene leads to persistent FPN1 expression on the cell membrane and subsequent iron overload [12, 13], and delivery of synthetic hepcidin results in decreased serum iron in response to diminished intestinal iron absorption . In sum, hepcidin dictates iron balance by restricting iron absorption and macrophage iron release, thereby reducing iron stores and affecting the availability of iron for conferring critical biological functions, including erythropoiesis .
Adding to the complexity of the maintenance of iron homeostasis, hepcidin synthesis is regulated by a number of factors, including iron status, inflammation, and erythropoiesis. The regulation of hepcidin by iron status appears to occur through the bone morphogenic protein (BMP) pathway. A number of BMPs, including BMP6, have been demonstrated to affect hepcidin production, in vivo [15–17]. For example, knockout of BMP6 in a mouse model results in severe iron overload [16, 18], and BMP6 expression increases with iron loading in mice . The regulation of hepcidin synthesis in response to inflammation occurs in response to proinflammatory cytokines, including interleukin-6 (IL-6). Increased circulating concentrations of IL-6, which may occur in response to inflammatory condition such as sepsis, inflammatory bowel disease, and obesity, stimulate hepcidin transcription through a STAT3-dependent mechanism [20–24]. Associations between iron status, inflammatory biomarkers, and hepcidin levels have been documented in recent human trials . Erythropoiesis, the biologic process requiring the greatest concentrations of body iron, is a suppressor of hepcidin production . Both human and animal studies have demonstrated reductions in serum hepcidin and mRNA levels, respectively, following erythropoietin administration [26, 27]. Although the finite mechanism by which erythropoiesis affects hepcidin production remains unknown, early evidence indicates that two proteins produced by erythroid precursors are involved in hepcidin regulation. Growth differentiation factor 15 (GDF15) has been demonstrated to suppress hepcidin expression and is elevated in patients with thalassemia . Another erythrokine, twisted gastrulation protein (TWSG1), has also been shown to suppress hepcidin mRNA in vitro .
Hypoxia is another factor that may affect hepcidin regulation, potentially through increased erythropoietin (EPO) production. The remainder of this paper will focus on potential mechanisms by which hypoxia may affect hepcidin levels, and, subsequently, iron homeostasis.
3. Hypoxia and Iron Metabolism
The profound effects of hypoxia on organismal iron metabolism have been well described. Early studies demonstrated that hypoxia affected dietary iron absorption [30–32] and increased erythropoiesis when humans were moved from sea level to high altitude . As the partial pressure of O2 decreases through increased elevation, anemia, or localized tissue hypoxia, a battery of genes are induced by the hypoxia inducible factor (HIF)/hypoxia response element (HRE) system. The HIF system senses O2 levels through degradation of HIF transcription factors (HIF-1α and HIF-2α) that are mediated by the partial pressure of O2 and iron-dependent hydroxylases. At normoxic O2 tensions, HIF-1α is hydroxylated by prolyl hydroxylase and then bound by the von Hippel-Lindau (VHL) protein leading ultimately to ubiquitination and proteasome degradation. During hypoxic conditions, the activity of hydroxylases is inhibited allowing HIF-1α to accumulate and bind along with HIF-1β to HRE found in the promoters of target genes. Similar to HIF-1α, HIF-2α stability is mediated by the partial pressure of O2 through prolyl hydroxylase and plays a dominant role in hypoxic signaling of EPO expression .
4. Hypoxia and Hepcidin Expression
Reduced expression of hepcidin during hypoxia was first reported in 2002 by Nicolas et al. , who demonstrated a marked reduction in hepcidin mRNA in HepG2 cells cultured at 2 and 0.1% O2 compared to standard 20% O2 culture conditions. Similarly, when mice were placed in atmospheric chambers emulating an altitude of 5,500 m, hepcidin expression was downregulated after 48 hrs of hypoxia. Leung et al.  also reported decreased hepcidin mRNA expression in HepG2 cells cultured at 1% O2 and in liver from rats placed in atmospheric chambers containing 10% O2. However, the molecular mechanisms linking hypoxia and decreased hepcidin expression remained unexplained. Because the HIF signaling system imparts a dominant role in sensing O2 deprivation and subsequent gene expression, including the upregulation of EPO , it is a probable, direct or upstream, mediator of hypoxia-induced, hepcidin gene regulation.
Peyssonnaux and colleagues  demonstrated that the expected hepcidin downregulation in response to dietary iron deficiency was partially blunted in hepatocyte-specific HIF-1α knockout mice as compared to wild-type animals, suggesting that HIF-1α might be a negative regulator of hepcidin signaling. Moreover, hepatocyte-specific VHL knockout mice have higher HIF-1 levels but reduced hepcidin expression and increased hepatic ferroportin levels, further suggesting a role for the HIF system as a negative hepcidin regulator. Peyssonnaux et al.  went on to demonstrate that murine and human hepcidin promoters contained a consensus HRE that could be immunoprecipitated by a HIF-1α antibody when cells were treated with the iron chelator and HIF-1α stabilizer desferrioxamine (DFO). Taken together, these data provided evidence that the HIF system is a negative regulator of hepcidin expression and provided a mechanism whereby hypoxia may decrease hepcidin expression. Typically, the HIF system functions as a positive regulator of gene expression ; however, negative transcriptional regulation by the HIF system is not unprecedented .
Studies by Braliou et al.  tested whether 2-oxoglutarate-dependent oxygenases mediated hepcidin regulation in response to hypoxia. Similar to studies by Nicolas et al.  and Leung et al. , hypoxia decreased hepcidin expression in both HepG2 and Huh7 cells. Hepcidin expression was also decreased by hypoxia mimetics, including dimethyloxaloylglycine (DMOG), DFO, and CoCl2. These mimetics function by stabilizing the HIF pathway by inhibiting 2-oxoglutarate-dependent oxygenases and thus preventing HIF-1α hydroxylation and destruction. While hypoxia, DFO, and CoCl2 only modestly decreased hepcidin expression, DMOG dramatically decreased hepcidin expression in both cell lines. Despite negative regulation by hypoxia and hypoxia mimetics, overexpression or knockdown of HIF-1α had no effect on hepcidin promoter activity suggesting that HIF-1α is not directly involved in hepcidin promoter transcriptional regulation. In a similar study by Choi et al. , hypoxia decreased hepcidin expression in both mice and HepG2 cells, and the cell culture studies indicated that decreased hepcidin expression was independent of HIF-1α. The authors concluded that hypoxia-induced oxidative stress decreased STAT3 and C/EBPα protein/DNA interactions, thus resulting in decreased hepcidin expression.
Interestingly, not all studies have demonstrated that hypoxia directly decreases in vitro hepcidin expression. In an investigation by Volke et al. , HepG2 and Huh7 cells cultured under hypoxic conditions did not have diminished hepcidin expression, contrary to previous reports [35, 36, 40]. However, similar to the study by Braliou et al. , the hypoxia mimetic DMOG, as well as the iron chelator 2,2′dipyridyl, decreased hepcidin expression. Moreover, Volke et al.  demonstrated that HIF-1α or HIF-1β knockdown did not increase hepcidin expression and concluded that reductions in hepcidin expression from hypoxia mimetics were not mediated through HIF. Also contrary to previous reports [35, 36, 38, 40, 41], Chaston et al.  reported that hypoxia increased Huh7 hepcidin expression. However, when Huh7 cells were cocultured with activated THP-1 macrophages, hypoxia reduced hepcidin expression. Using reporter constructs under control of the hepcidin promoter and the THP-1 coculture model, the investigators demonstrated that hypoxia-induced hepcidin repression in Huh7 cells was abrogated when the SMAD binding site was mutated. Mutations to the Ebox1,2, STAT3, and C/EBP sites had no effect, suggesting that hypoxia-mediated hepcidin regulation is controlled through the SMAD site and is dependent on macrophage coculture.
SMAD regulation of the hepcidin promoter is mediated through BMPs and bone morphogenetic response elements (BRE) in the hepcidin promoter. Binding of BMPs to their receptors causes phosphorylation of SMAD proteins, binding to the BRE and increased gene expression. The gene product of the HFE2 gene, hemojuvelin, is a coreceptor for BMP proteins, and overexpression increases hepcidin expression in liver cells . Regulation of hepcidin through BMP is independent of inflammatory signaling through STAT3, thus hepcidin may be regulated independently by different stimuli. It has been hypothesized that regulation of hepcidin by hemojuvelin is dependent upon whether hemojuvelin is membrane anchored or soluble. Membrane-anchored hemojuvelin is a positive regulator of the BMP/SMAD cascade, whereas soluble hemojuvelin functions as a dummy receptor, inhibits the pathway, and results in reduced hepcidin expression .
Several studies have provided evidence that hepcidin may be regulated by hypoxia through the hemojuvelin/BMP axis and hypoxic regulation of the hemojuvelin cleaving proteins matripase-2 (TMPRSS6) and furin. Du et al.  first reported that mice with a homozygous TMPRSS6 mutation, referred to as mask, have compromised iron status due to excessive hepcidin signaling. Moreover, hepcidin promoter activation by cotransfection of a hemojuvelin expression construct or recombinant BMP2, BMP4, or BMP9 proteins was blunted when cells were treated with a wild-type TMPRSS6 expression vector but not with a vector coding for TMPRSS6 with the mask mutation. Folgueras et al.  and Finberg et al.  further demonstrated the importance of TMPRSS6 on hepcidin regulation by demonstrating that TMPRSS6 knockout mice have elevated hepcidin levels and decreased iron stores compared to wild-type mice. Furthermore, TMPRSS6 mutations have been described in humans and result in aberrant hepcidin signaling and iron-refractory iron-deficiency anemia [48–51]. TMPRSS6 was demonstrated by Silvestri et al.  to cleave membrane hemojuvelin and inhibit hepcidin expression. The same research group  also reported that hemojuvelin can be cleaved by furin in the endoplasmic reticulum. Furin promoter activity was increased by the HIF stabilizers DFO and CoCl2 and decreased by ferric ammonium citrate treatment suggesting HIF regulation. This is supported by an earlier study demonstrating that furin expression is positively regulated by hypoxia via the HIF pathway .
Recently, Lakhal et al.  demonstrated that TMPRSS6 is regulated by hypoxia through HIF-1α and 2α. TMPRSS6 gene expression increased in Hep3B cells when cultured at 0.5% O2 and in the presence of the hypoxia mimetic DMOG. Increased TMPRSS6 expression induced by hypoxia or hypoxia mimetics was curtailed by siRNA knockdown of HIF-1α and/or 2α. It was also reported that hypoxia reduced membrane hemojuvelin and this effect could be abrogated by siRNA knockdown of TMPRSS6, HIF-1α, and 2α. Hypoxia decreased activity of luciferase constructs containing the proximal hepcidin BRE, and this effect was inhibited when cells were treated with either TMPRSS6 or HIF-1α and 2α siRNA or if the promoter BRE was mutated.
5. Localized Adipose Hypoxia and Hepcidin Expression
Early studies have demonstrated a link between obesity and poor iron status [56, 57]. Studies have demonstrated this association in children and adolescents [57–60], adult men and women [61–64], and postmenopausal women . Obesity has been suggested as an independent factor contributing to iron deficiency [60, 61, 63, 65], and studies have correlated obesity, BMI, and increased hepcidin in premenopausal women  and children . Chronic inflammation is associated with central obesity and has been implicated in many obesity-related problems such as insulin resistance [68, 69]. Obese mice (ob/ob line) have significantly higher plasma IL-6 levels than their lean counterparts, and this difference is attributed to increased IL-6 expression in adipose tissue, suggesting that adipose tissue contributes to plasma inflammatory cytokine levels .
Adipose hypoxia is a contributing factor to increased inflammation associated with obesity. Ye et al.  demonstrated that adipose tissue in ob/ob mice is considerably more hypoxic than adipose tissue from lean mice. Differences in O2 tension in ob/ob mice increased the expression of the inflammatory cytokines TNF-α, IL-1, and IL-6. Increased expression of cytokines was also demonstrated in 3T3-L1 cells cultured under hypoxic conditions (1% O2), suggesting that the phenomenon of obesity-induced inflammation may result from increased secretion of inflammatory cytokines from hypoxic adipocytes found in deep fat pockets. Another consequence of increased adiposity and adipocyte hypoxia is macrophage infiltration of adipose tissue. Low partial pressure of O2 in human adipose tissue is correlated to macrophage infiltration [72, 73]. Several studies have demonstrated that obesity in humans increases markers of adipose macrophage content and subsequent inflammation [73, 74].
Hepcidin expression is well known to be induced by inflammation. IL-6 mediates hepcidin induction by increasing the binding of the transcription factor STAT3 to hepcidin promoter [20–22]. Considering that central obesity and resulting adipocyte hypoxia increase systemic IL-6 , it stands to reason that adipocyte hypoxia may be involved in the hypoferremia of obesity through increased inflammatory signaling of hepcidin. Hintze et al.  recently demonstrated that Huh7 hepatocytes treated with conditioned media from 3T3-L1 adipocytes cultured at 1% O2 had significantly greater hepcidin promoter activity and mRNA levels compared to Huh7 cells treated with conditioned media from 3T3-L1 cultured at 21% O2. Hypoxic adipocytes had increased levels of both IL-6 and leptin mRNA. Leptin and IL-6 have been demonstrated to induce hepcidin expression via the STAT3 pathway [75, 76] suggesting a possible mechanism whereby adipocyte hypoxia may signal hepcidin. When the hepcidin STAT3 binding site was mutated in the hepcidin promoter, activity was diminished, suggesting that adipocyte hypoxia may increase hepcidin expression through increased inflammation.
The recent discovery of hepcidin, which has been described as a central regulator of iron homeostasis, has provided evidence that iron balance is a tightly regulated process affected by a series of factors other than diet, to include hypoxia. Hypoxia has profound effects on iron absorption [30–32] and results in increased erythropoiesis when humans move from sea level to altitude . Several mechanisms or combinations thereof may explain decreased hepcidin expression mediated by hypoxia (Figure 2). There is evidence that the hepcidin promoter may be a target for direct negative regulation by the HIF/HRE system , although not all studies support this assertion . Despite sometimes conflicting results, studies utilizing hypoxia mimetics that stabilize HIF proteins have consistently demonstrated decreased hepcidin expression [38, 40, 42, 55] suggesting that the HIF system is involved in hepcidin regulation either directly or through upstream mediators. Localized adipose hypoxia due to obesity may in part play a role in hepcidin signaling and organismal iron metabolism. The emerging literature concerning obesity, adipocyte hypoxia, and inflammation suggests that the etiology of obesity-related hypoferremia may be mediated in part through increased hepcidin expression through inflammatory pathways originating from adipose tissue. Research in the area of hypoxia and iron metabolism continues to provide novel evidence of the molecular regulation of hepcidin and its effects on iron status. It is likely that recent findings will result in the development of novel interventions affecting hepcidin expression, and, subsequently, iron balance.
The authors acknowledge Ms. Nancy Andersen for her technical contribution to the construction of this paper and Dr. Andrew Young for his critical review. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Army or the Department of Defense. Any citations of commercial organizations and trade names in this paper do not constitute an official Department of the Army endorsement of approval of the products or services of these organizations.
- A. C. Looker, M. E. Cogswell, and M. T. Gunter, “Iron deficiency—United States, 1999–2000,” Morbidity and Mortality Weekly Report, vol. 51, no. 40, pp. 897–899, 2002.
- K. J. Davies, J. J. Maguire, G. A. Brooks, P. R. Dallman, and L. Packer, “Muscle mitochondrial bioenergetics, oxygen supply, and work capacity during dietary iron deficiency and repletion,” The American Journal of Physiology, vol. 242, no. 6, pp. E418–427, 1982.
- P. R. Dallman, “Biochemical basis for the manifestations of iron deficiency,” Annual Review of Nutrition, vol. 6, pp. 13–40, 1986.
- J. P. McClung, J. P. Karl, S. J. Cable et al., “Randomized, double-blind, placebo-controlled trial of iron supplementation in female soldiers during military training: effects on iron status, physical performance, and mood,” American Journal of Clinical Nutrition, vol. 90, no. 1, pp. 124–131, 2009.
- R. Yip and P. R. Dallman, “Iron,” in Present Knowledge in Nutrition, ILSI Press, Washington, DC, USA, 1996.
- A. Krause, S. Neitz, H. J. Mägert et al., “LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity,” FEBS Letters, vol. 480, no. 2-3, pp. 147–150, 2000.
- C. H. Park, E. V. Valore, A. J. Waring, and T. Ganz, “Hepcidin, a urinary antimicrobial peptide synthesized in the liver,” Journal of Biological Chemistry, vol. 276, no. 11, pp. 7806–7810, 2001.
- T. Ganz, “Hepcidin and its role in regulating systemic iron metabolism,” Hematology, vol. 507, pp. 29–35, 2006.
- C. Pigeon, G. Ilyin, B. Courselaud et al., “A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload,” Journal of Biological Chemistry, vol. 276, no. 11, pp. 7811–7819, 2001.
- I. de Domenico, D. M. Ward, M. C. B. Di Patti et al., “Ferroxidase activity is required for the stability of cell surface ferroportin in cells expressing GPI-ceruloplasmin,” EMBO Journal, vol. 26, no. 12, pp. 2823–2831, 2007.
- M. D. Fleming, “The regulation of hepcidin and its effects on systemic and cellular iron metabolism,” Hematology, pp. 151–158, 2008.
- G. Nicolas, M. Bennoun, I. Devaux et al., “Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 15, pp. 8780–8785, 2001.
- L. Viatte, J. C. Lesbordes-Brion, D. Q. Lou et al., “Deregulation of proteins involved in iron metabolism in hepcidin-deficient mice,” Blood, vol. 105, no. 12, pp. 4861–4864, 2005.
- A. H. Laftah, B. Ramesh, R. J. Simpson et al., “Effect of hepcidin on intestinal iron absorption in mice,” Blood, vol. 103, no. 10, pp. 3940–3944, 2004.
- Y. Xia, J. L. Babitt, Y. Sidis, R. T. Chung, and H. Y. Lin, “Hemojuvelin regulates hepcidin expression via a selective subset of BMP ligands and receptors independently of neogenin,” Blood, vol. 111, no. 10, pp. 5195–5204, 2008.
- B. Andriopoulos Jr., E. Corradini, Y. Xia et al., “BMP6 is a key endogenous regulator of hepcidin expression and iron metabolism,” Nature Genetics, vol. 41, no. 4, pp. 482–487, 2009.
- J. L. Babitt, F. W. Huang, D. M. Wrighting et al., “Bone morphogenetic protein signaling by hemojuvelin regulates hepcidin expression,” Nature Genetics, vol. 38, no. 5, pp. 531–539, 2006.
- D. Meynard, L. Kautz, V. Darnaud, F. Canonne-Hergaux, H. Coppin, and M. P. Roth, “Lack of the bone morphogenetic protein BMP6 induces massive iron overload,” Nature Genetics, vol. 41, no. 4, pp. 478–481, 2009.
- L. Kautz, D. Meynard, A. Monnier et al., “Iron regulates phosphorylation of Smad1/5/8 and gene expression of Bmp6, Smad7, Id1, and Atoh8 in the mouse liver,” Blood, vol. 112, no. 4, pp. 1503–1509, 2008.
- D. M. Wrighting and N. C. Andrews, “Interleukin-6 induces hepcidin expression through STAT3,” Blood, vol. 108, no. 9, pp. 3204–3209, 2006.
- M. V. Verga Falzacappa, M. V. Spasic, R. Kessler, J. Stolte, M. W. Hentze, and M. U. Muckenthaler, “STAT3 mediates hepatic hepcidin expression and its inflammatory stimulation,” Blood, vol. 109, no. 1, pp. 353–358, 2007.
- E. Nemeth, S. Rivera, V. Gabayan et al., “IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin,” Journal of Clinical Investigation, vol. 113, no. 9, pp. 1271–1276, 2004.
- J. P. McClung and J. P. Karl, “Iron deficiency and obesity: the contribution of inflammation and diminished iron absorption,” Nutrition Reviews, vol. 67, no. 2, pp. 100–104, 2009.
- E. Nemeth, “Targeting the hepcidin-ferroportin axis in the diagnosis and treatment of anemias,” Advances in Hematology, vol. 2010, Article ID 750643, 9 pages, 2010.
- J. P. Karl, H. R. Lieberman, S. J. Cable, K. W. Williams, A. J. Young, and J. P. McClung, “Randomized, double-blind, placebo-controlled trial of an iron-fortified food product in female soldiers during military training: relations between iron status, serum hepcidin, and inflammation,” The American Journal of Clinical Nutrition, vol. 92, no. 1, pp. 93–100, 2010.
- H. Huang, M. Constante, A. Layoun, and M. M. Santos, “Contribution of STAT3 and SMAD4 pathways to the regulation of hepcidin by opposing stimuli,” Blood, vol. 113, no. 15, pp. 3593–3599, 2009.
- D. R. Ashby, D. P. Gale, M. Busbridge et al., “Erythropoietin administration in humans causes a marked and prolonged reduction in circulating hepcidin,” Haematologica, vol. 95, no. 3, pp. 505–508, 2010.
- T. Tanno, N. V. Bhanu, P. A. Oneal et al., “High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin,” Nature Medicine, vol. 13, no. 9, pp. 1096–1101, 2007.
- T. Tanno, P. Porayette, O. Sripichai et al., “Identification of TWSG1 as a second novel erythroid regulator of hepcidin expression in murine and human cells,” Blood, vol. 114, no. 1, pp. 181–186, 2009.
- D. M. Taylor and P. S. Vassar, “Effects of hypoxia on iron absorption in rats,” Proceedings of the Society for Experimental Biology and Medicine, vol. 93, no. 3, pp. 504–506, 1956.
- S. Krantz, E. Goldwasser, and L. O. Jacobson, “Studies on erythropoiesis. XIV. The relationship of humoral stimulation to iron absorption,” Blood, vol. 14, no. 6, pp. 654–661, 1959.
- G. W. Strohmeyer, S. A. Miller, R. W. Scarlata, E. W. Moore, M. S. Greenberg, and T. C. Chalmers, “Effects of hypoxia on iron absorption and mobilization in the rat,” The American Journal of Physiology, vol. 207, pp. 55–61, 1964.
- C. Reynafarje, R. Lozano, and J. Valdivieso, “The polycythemia of high altitudes: iron metabolism and related aspects,” Blood, vol. 14, no. 4, pp. 433–455, 1959.
- G. L. Semenza, “Involvement of oxygen-sensing pathways in physiologic and pathologic erythropoiesis,” Blood, vol. 114, no. 10, pp. 2015–2019, 2009.
- G. Nicolas, C. Chauvet, L. Viatte et al., “The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation,” Journal of Clinical Investigation, vol. 110, no. 7, pp. 1037–1044, 2002.
- P. S. Leung, S. K. Srai, M. Mascarenhas, L. J. Churchill, and E. S. Debnam, “Increased duodenal iron uptake and transfer in a rat model of chronic hypoxia is accompanied by reduced hepcidin expression,” Gut, vol. 54, no. 10, pp. 1391–1395, 2005.
- G. L. Semenza, “Regulation of mammalian O2 homeostasis by hypoxia-inducible factor 1,” Annual Review of Cell and Developmental Biology, vol. 15, pp. 551–578, 1999.
- C. Peyssonnaux, A. S. Zinkernagel, R. A. Schuepbach et al., “Regulation of iron homeostasis by the hypoxia-inducible transcription factors (HIFs),” Journal of Clinical Investigation, vol. 117, no. 7, pp. 1926–1932, 2007.
- N. M. Mazure, C. Chauvet, B. Bois-Joyeux, M. A. Bernard, H. Nacer-Chérif, and J. L. Danan, “Repression of α-fetoprotein gene expression under hypoxic conditions in human hepatoma cells: characterization of a negative hypoxia response element that mediates opposite effects of hypoxia inducible factor-1 and c-Myc,” Cancer Research, vol. 62, no. 4, pp. 1158–1165, 2002.
- G. G. Braliou, M. V. Verga Falzacappa, G. Chachami, G. Casanovas, M. U. Muckenthaler, and G. Simos, “2-Oxoglutarate-dependent oxygenases control hepcidin gene expression,” Journal of Hepatology, vol. 48, no. 5, pp. 801–810, 2008.
- S. O. Choi, Y. S. Cho, H. L. Kim, and J. W. Park, “ROS mediate the hypoxic repression of the hepcidin gene by inhibiting C/EBPα and STAT-3,” Biochemical and Biophysical Research Communications, vol. 356, no. 1, pp. 312–317, 2007.
- M. Volke, D. P. Gale, U. Maegdefrau et al., “Evidence for a lack of a direct transcriptional suppression of the iron regulatory peptide hepcidin by hypoxia-inducible factors,” PLoS ONE, vol. 4, no. 11, Article ID e7875, 2009.
- T. B. Chaston, P. Matak, K. Pourvali, S. K. Srai, A. T. McKie, and P. A. Sharp, “Hypoxia inhibits hepcidin expression in HuH7 hepatoma cells via decreased SMAD4 signaling,” American Journal of Physiology, vol. 300, no. 4, pp. C888–C895, 2011.
- L. Lin, Y. P. Goldberg, and T. Ganz, “Competitive regulation of hepcidin mRNAby soluble and cell-associated hemojuvelin,” Blood, vol. 106, no. 8, pp. 2884–2889, 2005.
- X. Du, E. She, T. Gelbart et al., “The serine protease TMPRSS6 is required to sense iron deficiency,” Science, vol. 320, no. 5879, pp. 1088–1092, 2008.
- A. R. Folgueras, F. M. de Lara, A. M. Pendás et al., “Membrane-bound serine protease matriptase-2 (Tmprss6) is an essential regulator of iron homeostasis,” Blood, vol. 112, no. 6, pp. 2539–2545, 2008.
- K. E. Finberg, R. L. Whittlesey, M. D. Fleming, and N. C. Andrews, “Down-regulation of Bmp/Smad signaling by Tmprss6 is required for maintenance of systemic iron homeostasis,” Blood, vol. 115, no. 18, pp. 3817–3826, 2010.
- K. E. Finberg, M. M. Heeney, D. R. Campagna et al., “Mutations in TMPRSS6 cause iron-refractory iron deficiency anemia (IRIDA),” Nature Genetics, vol. 40, no. 5, pp. 569–571, 2008.
- L. Silvestri, F. Guillem, A. Pagani et al., “Molecular mechanisms of the defective hepcidin inhibition in TMPRSS6 mutations associated with iron-refractory iron deficiency anemia,” Blood, vol. 113, no. 22, pp. 5605–5608, 2009.
- M. A. Melis, M. Cau, R. Congiu et al., “A mutation in the TMPRSS6 gene, encoding a transmembrane serine protease that suppresses hepcidin production, in familial iron deficiency anemia refractory to oral iron,” Haematologica, vol. 93, no. 10, pp. 1473–1479, 2008.
- F. Guillem, S. Lawson, C. Kannengiesser, M. Westerman, C. Beaumont, and B. Grandchamp, “Two nonsense mutations in the TMPRSS6 gene in a patient with microcytic anemia and iron deficiency,” Blood, vol. 112, no. 5, pp. 2089–2091, 2008.
- L. Silvestri, A. Pagani, A. Nai, I. de Domenico, J. Kaplan, and C. Camaschella, “The serine protease matriptase-2 (TMPRSS6) inhibits hepcidin activation by cleaving membrane hemojuvelin,” Cell Metabolism, vol. 8, no. 6, pp. 502–511, 2008.
- L. Silvestri, A. Pagani, and C. Camaschella, “Furin-mediated release of soluble hemojuvelin: a new link between hypoxia and iron homeostasis,” Blood, vol. 111, no. 2, pp. 924–931, 2008.
- S. McMahon, F. Grondin, P. P. McDonald, D. E. Richard, and C. M. Dubois, “Hypoxia-enhanced expression of the proprotein convertase furin is mediated by hypoxia-inducible factor-1: impact on the bioactivation of proproteins,” Journal of Biological Chemistry, vol. 280, no. 8, pp. 6561–6569, 2005.
- S. Lakhal, J. Schödel, A. R. Townsend, C. W. Pugh, P. J. Ratcliffe, and D. R. Mole, “Regulation of type II transmembrane serine proteinase TMPRSS6 by hypoxia-inducible factors: new link between hypoxia signaling and iron homeostasis,” Journal of Biological Chemistry, vol. 286, no. 6, pp. 4090–4097, 2011.
- B. Wenzel, H. Stults, and J. Mayer, “Hypoferraemia in obese adolescents,” The Lancet, vol. 2, no. 7251, pp. 327–328, 1962.
- C. C. Seltzer and J. Mayer, “Serum iron and iron-binding capacity in adolescents. II. Comparison of obese and nonobese subjects,” The American Journal of Clinical Nutrition, vol. 13, pp. 354–361, 1963.
- K. G. Nead, J. S. Halterman, J. M. Kaczorowski, P. Auinger, and M. Weitzman, “Overweight children and adolescents: a risk group for iron deficiency,” Pediatrics, vol. 114, no. 1, pp. 104–108, 2004.
- O. Pinhas-Hamiel, R. S. Newfield, I. Koren, A. Agmon, P. Lilos, and M. Phillip, “Greater prevalence of iron deficiency in overweight and obese children and adolescents,” International Journal of Obesity, vol. 27, no. 3, pp. 416–418, 2003.
- L. M. Tussing-Humphreys, H. Liang, E. Nemeth, S. Freels, and C. A. Braunschweig, “Excess adiposity, inflammation, and iron-deficiency in female adolescents,” Journal of the American Dietetic Association, vol. 109, no. 2, pp. 297–302, 2009.
- K. J. Ausk and G. N. Ioannou, “Is obesity associated with anemia of chronic disease? A population-based study,” Obesity, vol. 16, no. 10, pp. 2356–2361, 2008.
- C. M. Menzie, L. B. Yanoff, B. I. Denkinger et al., “Obesity-related hypoferremia is not explained by differences in reported intake of heme and nonheme iron or intake of dietary factors that can affect iron absorption,” Journal of the American Dietetic Association, vol. 108, no. 1, pp. 145–148, 2008.
- L. B. Yanoff, C. M. Menzie, B. Denkinger et al., “Inflammation and iron deficiency in the hypoferremia of obesity,” International Journal of Obesity, vol. 31, no. 9, pp. 1412–1419, 2007.
- E. C. Chambers, S. Heshka, D. Gallagher, J. Wang, F. X. Pi-Sunyer, and R. N. Pierson Jr., “Serum iron and body fat distribution in a multiethnic cohort of adults living in New York City,” Journal of the American Dietetic Association, vol. 106, no. 5, pp. 680–684, 2006.
- A. Lecube, A. Carrera, E. Losada, C. Hernández, R. Simó, and J. Mesa, “Iron deficiency in obese postmenopausal women,” Obesity, vol. 14, no. 10, pp. 1724–1730, 2006.
- L. M. Tussing-Humphreys, E. Nemeth, G. Fantuzzi et al., “Elevated systemic hepcidin and iron depletion in obese premenopausal females,” Obesity, vol. 18, no. 7, pp. 1449–1456, 2010.
- I. Aeberli, R. F. Hurrell, and M. B. Zimmermann, “Overweight children have higher circulating hepcidin concentrations and lower iron status but have dietary iron intakes and bioavailability comparable with normal weight children,” International Journal of Obesity, vol. 33, no. 10, pp. 1111–1117, 2009.
- B. B. Kahn and J. S. Flier, “Obesity and insulin resistance,” Journal of Clinical Investigation, vol. 106, no. 4, pp. 473–481, 2000.
- G. S. Hotamisligil, P. Arner, J. F. Caro, R. L. Atkinson, and B. M. Spiegelman, “Increased adipose tissue expression of tumor necrosis factor-α in human obesity and insulin resistance,” Journal of Clinical Investigation, vol. 95, no. 5, pp. 2409–2415, 1995.
- J. M. Harkins, N. Moustaid-Moussa, Y. J. Chung et al., “Expression of interleukin-6 is greater in preadipocytes than in adipocytes of 3T3-L1 cells and C57BL/6J and ob/ob mice,” Journal of Nutrition, vol. 134, no. 10, pp. 2673–2677, 2004.
- J. Ye, Z. Gao, J. Yin, and Q. He, “Hypoxia is a potential risk factor for chronic inflammation and adiponectin reduction in adipose tissue of ob/ob and dietary obese mice,” American Journal of Physiology, vol. 293, no. 4, pp. E1118–E1128, 2007.
- M. Pasarica, O. R. Sereda, L. M. Redman et al., “Reduced adipose tissue oxygenation in human obesity evidence for rarefaction, macrophage chemotaxis, and inflammation without an angiogenic response,” Diabetes, vol. 58, no. 3, pp. 718–725, 2009.
- K. H. Pietiläinen, K. Kannisto, E. Korsheninnikova et al., “Acquired obesity increases CD68 and tumor necrosis factor-α and decreases adiponectin gene expression in adipose tissue: a study in monozygotic twins,” Journal of Clinical Endocrinology and Metabolism, vol. 91, no. 7, pp. 2776–2781, 2006.
- J. Makkonen, J. Westerbacka, M. Kolak et al., “Increased expression of the macrophage markers and of 11β-HSD-1 in subcutaneous adipose tissue, but not in cultured monocyte-derived macrophages, is associated with liver fat in human obesity,” International Journal of Obesity, vol. 31, no. 10, pp. 1617–1625, 2007.
- K. J. Hintze, D. Snow, D. Nabor, and H. Timbimboo, “Adipocyte hypoxia increases hepatocyte hepcidin expression,” Biological Trace Element Research. In press.
- B. Chung, P. Matak, A. T. McKie, and P. Sharp, “Leptin increases the expression of the iron regulatory hormone hepcidin in HuH7 human hepatoma cells,” Journal of Nutrition, vol. 137, no. 11, pp. 2366–2370, 2007.