Through the Looking Glass: Visualizing Leukemia Growth, Migration, and Engraftment Using Fluorescent Transgenic Zebrafish

Figure 2

Zebrafish T-lymphoblasts overexpressing bcl2 spread locally but fail to intravasate into vasculature. (a)–(c) dsRED2-expressing lymphoma cells (b) from the Myc; Cre fish intravasate into EGFP-labeled vasculature (a) of the transplant host Tg(fli1:EGFP); Casper by 6 days posttransplantation (see arrowheads in (c)). (d)–(f) In contrast, dsRED2-expressing lymphoma cells (e) from the Myc; Cre; bcl2 fish fail to intravasate vasculature (d) of the transplant hosts by 6 days posttransplantation (compare (f) with (c)). Note aggregates of the Myc; Cre; bcl2 lymphoma cells in (e) and (f). Scale bar is 10 μm. Reprinted from [10].