Figure 2: Effect of transfusion of fresh and aged RBC on systemic inflammation response and lung endothelial permeability. Twenty-four hours after major blood loss (0.02 mL/gm), mice were administered 0.3 mL of PBS (“vehicle”), freshly isolated RBC at 50% Hct (“fresh RBC”), or RBC stored for >14 days (“aged RBC”) at 50% Hct. Fortyeight hours after initial blood loss, (a) Hct, (b) plasma Il-6, (c) plasma KC, (d) white blood cell count (WBC), (e) blood S1P, and (f) lung endothelial permeability were measured. IL-6, KC, and S1P are reported as % of baseline (time 0). Results were analyzed by one-way ANOVA (Bonferroni correction),* versus vehicle.