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Figure 3: Effect of anemia on LPS-induced systemic inflammation and lung endothelial permeability. At time = 0 (baseline), mice were randomized in a 2 : 1 fashion to major blood loss (0.02 mL/gm) or minor blood collection (0.002 mL/gm). Twentyfour hours later, mice that had undergone major blood loss (“blood loss”) were given vehicle or low dose LPS (2 mg/kg, i.p.). Mice that had undergone minor blood loss were given LPS alone. (a) Hct was measured at 48 hours after initial blood loss ( mice/group). (b) Plasma Il-6 and (c) plasma KC were measured at 28 hours or 48 hours after initial blood loss ( mice/group). (d) Blood S1P was measured at 28 hours. (e) Quantification of EBD in the lungs, as a marker for endothelial permeability, was performed at 48 hours after initial blood loss ( /group). (f) Representative images of EBD in the lungs after the indicated treatments. Results were analyzed by one-way ANOVA (Bonferroni correction); * versus vehicle.