RhD Specific Antibodies Are Not Detectable in HLA-DRB1 Mice Challenged with Human RhD Positive Erythrocytes

Figure 3

Immunization of DRB1 mice with human RhD positive RBCs induces RBC-reactive but not RhD-specific IgG as detected by flow cytometry. DRB1 mice were challenged with one () or two doses of 10⁸ RhD positive RBC () or 1 dose of 10⁸ RhD positive RBC emulsified in CpG ODN adjuvant (). Untreated mice were used as negative controls (Nil) (). (a) Detection of IgG reactive with human RhD positive RBC. (b) Detection of IgG reactive with human RhD positive RBC after adsorbing the sera with RhD negative cells. MFI: mean fluorescence intensity.

(a)

(b)