Review Article
Transplantation of Encapsulated Pancreatic Islets as a Treatment for Patients with Type 1 Diabetes Mellitus
Table 1
Enzyme types used for human islet isolation.
| Enzyme types | Manufacture | Concentration | Digestion time (min) |
| Sigma type V collagenase | Sigma | 1 g/350 mL | 14 | BM Type P collagenase | Boehringer-Mannheim | 0.7 g/350 mL | 25 | Liberase HI | Roche Applied Science | 0.5 g/350 mL | 16 | Liberase MTF C/T, GMP grade | Roche Applied Science | | 19 | Collagenase | | 0.5 g/350 mL | | Thermolysin | | 0.015 g/350 mL | | Collagenase NB 1 (premium and GMP) | SERVA Electrophoresis GmbH | | 14 | Collagenase | | 1600–2286 PZ units/350 mL | | Neutral Protease | | 200–286 DMC units/350 mL | | VitaCyte C1 collagenase | VitaCyte | | 20 | CIzyme collagenase HA | | 15–18 units/g tissue | | CIzyme thermolysin | | 1.25 DMC units/g tissue | |
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MTF: mammalian tissue free. GMP: good manufacturing practice. DMC: dimethylcasein.
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