Transplantation of Encapsulated Pancreatic Islets as a Treatment for Patients with Type 1 Diabetes Mellitus

Table 1

Enzyme types used for human islet isolation.

Enzyme types	Manufacture	Concentration	Digestion time; (min)
Sigma type V collagenase	Sigma	1 g/350 mL	14
BM Type P collagenase	Boehringer-Mannheim	0.7 g/350 mL	25
Liberase HI	Roche Applied Science	0.5 g/350 mL	16
Liberase MTF C/T, GMP grade	Roche Applied Science		19
Collagenase		0.5 g/350 mL
Thermolysin		0.015 g/350 mL
Collagenase NB 1 (premium and GMP)	SERVA Electrophoresis GmbH		14
Collagenase		1600–2286 PZ units/350 mL
Neutral Protease		200–286 DMC units/350 mL
VitaCyte C1 collagenase	VitaCyte		20
CIzyme collagenase HA		15–18 units/g tissue
CIzyme thermolysin		1.25 DMC units/g tissue

MTF: mammalian tissue free.
GMP: good manufacturing practice.
DMC: dimethylcasein.