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Advances in Materials Science and Engineering
Volume 2013 (2013), Article ID 140643, 6 pages
http://dx.doi.org/10.1155/2013/140643
Research Article

Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications

1Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan
2Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan
3Department of Oral Biomaterials and Technology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
4Department of Dental Caries and Aesthetic Dentistry, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan

Received 26 December 2012; Accepted 10 May 2013

Academic Editor: Peter Majewski

Copyright © 2013 Toshihiro Inami et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This work describes the fabrication, optimization, and characterization of electrospun fibrous poly(D,L-lactic acid) (PDLLA) for biomedical applications. The influences of the polymer concentration of the electrospinning solution (5, 10, or 15 wt%) and the solution flow rate (0.1, 0.5, 1.0, or 2.0 mL/h) on the morphology of the obtained fibrous PDLLA were evaluated. The in vitro biocompatibility of two types of PDLLA, ester terminated PDLLA (PDLLA-R) and carboxyl terminated PDLLA (PDLLA-COOH), was evaluated by monitoring apatite formation on samples immersed in Hanks’ balanced salt (HBS) solution. 15 wt% polymer solution was the most beneficial for preparing a fibrous PDLLA structure. Meanwhile, no differences in morphology were observed for PDLLA prepared at various flow rates. Apatite precipitate is formed on both types of PDLLA only 1 day after immersion in HBS solution. After 7 days of immersion, PDLLA-COOH showed greater apatite formation ability compared with that of PDLLA-R, as measured by thin-film X-ray diffraction. The results indicated that the carboxyl group is effective for apatite precipitation in the body environment.