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Anemia
Volume 2011 (2011), Article ID 945289, 8 pages
http://dx.doi.org/10.1155/2011/945289
Research Article

Uptake of Non-Transferrin Iron by Erythroid Cells

Department of Hematology, Hadassah-Hebrew University Medical Center, Ein-Kerem, P.O. Box 12000, Jerusalem 91120, Israel

Received 20 September 2010; Accepted 7 November 2010

Academic Editor: Antonis Kattamis

Copyright © 2011 Eugenia Prus and Eitan Fibach. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. D. R. Richardson and P. Ponka, “The molecular mechanisms of the metabolism and transport of iron in normal and neoplastic cells,” Biochimica et Biophysica Acta, vol. 1331, no. 1, pp. 1–40, 1997. View at Publisher · View at Google Scholar · View at Scopus
  2. M. D. Fleming, C. C. Trenor, M. A. Su et al., “Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene,” Nature Genetics, vol. 16, no. 4, pp. 383–386, 1997. View at Scopus
  3. Z. I. Cabantchik, OR. Kakhlon, S. Epsztejn, G. Zanninelli, and W. Breuer, “Intracellular and extracellular labile iron pools,” Advances in Experimental Medicine and Biology, vol. 509, pp. 55–75, 2003. View at Scopus
  4. Z. I. Cabantchik, W. Breuer, G. Zanninelli, and P. Cianciulli, “LPI-labile plasma iron in iron overload,” Best Practice and Research: Clinical Haematology, vol. 18, no. 2, pp. 277–287, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  5. A. Jacobs, “Low molecular weight intracellular iron transport compounds,” Blood, vol. 50, no. 3, pp. 433–439, 1977. View at Scopus
  6. E. Fibach and E. Rachmilewitz, “The role of oxidative stress in hemolytic anemia,” Current Molecular Medicine, vol. 8, no. 7, pp. 609–619, 2008. View at Publisher · View at Google Scholar · View at Scopus
  7. E. Prus and E. Fibach, “The labile iron pool in human erythroid cells,” British Journal of Haematology, vol. 142, no. 2, pp. 301–307, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  8. E. H. Morgan, “Membrane transport of non-transferrin-bound iron by reticulocytes,” Biochimica et Biophysica Acta, vol. 943, no. 3, pp. 428–439, 1988. View at Scopus
  9. A. Egyed, “Carrier mediated iron transport through erythroid cell membrane,” British Journal of Haematology, vol. 68, no. 4, pp. 483–486, 1988. View at Scopus
  10. L. M. Garrick, K. G. Dolan, M. A. Romano, and M. D. Garrick, “Non-transferrin-bound iron uptake in Belgrade and normal rat erythroid cells,” Journal of Cellular Physiology, vol. 178, no. 3, pp. 349–358, 1999. View at Scopus
  11. F. Canonne-Hergaux, AN. S. Zhang, P. Ponka, and P. Gros, “Characterization of the iron transporter DMT1 (NRAMP/DCT) in red blood cells of normal and anemic mk/mk mice,” Blood, vol. 98, no. 13, pp. 3823–3830, 2001. View at Publisher · View at Google Scholar · View at Scopus
  12. E. Prus and E. Fibach, “Flow cytometry measurement of the labile iron pool in human hematopoietic cells,” Cytometry Part A, vol. 73, no. 1, pp. 22–27, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  13. P. Ramos, L. Melchiori, S. Gardenghi et al., “Iron metabolism and ineffective erythropoiesis in β-thalassemia mouse models,” Annals of the New York Academy of Sciences, vol. 1202, pp. 24–30, 2010. View at Publisher · View at Google Scholar · View at PubMed
  14. E. Fibach, D. Manor, A. Oppenheim, and E. A. Rachmilewitz, “Proliferation and maturation of human erythroid progenitors in liquid culture,” Blood, vol. 73, no. 1, pp. 100–103, 1989. View at Scopus
  15. E. Fibach and E. Prus, “Differentiation of human erythroid cells in culture,” in Current Protocols in Immunology, J. Coligan, B. Bierer, D. Margulies, E. Shevach, and W. Strober, Eds., vol. 2, supplement 69, pp. 22.F7.1–22.F7.10, John Wiley & Sons, Edison, NJ, USA, 2005.
  16. S. A. Weston and C. R. Parish, “New fluorescent dyes for lymphocyte migration studies: analysis by flow cytometry and fluorescence microscopy,” Journal of Immunological Methods, vol. 133, no. 1, pp. 87–97, 1990. View at Publisher · View at Google Scholar · View at Scopus
  17. S. A. Weston and C. R. Parish, “Calcein: a novel marker for lymphocytes which enter lymph nodes,” Cytometry, vol. 13, no. 7, pp. 739–749, 1992. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  18. W. Breuer, S. Epsztejn, and Z. I. Cabantchik, “Iron acquired from transferrin by K562 cells is delivered into a cytoplasmic pool of chelatable iron(II),” Journal of Biological Chemistry, vol. 270, no. 41, pp. 24209–24215, 1995. View at Publisher · View at Google Scholar · View at Scopus
  19. F. Petrat, D. Weisheit, M. Lensen, H. De Groot, R. Sustmann, and U. Rauen, “Selective determination of mitochondrial chelatable iron in viable cells with a new fluorescent sensor,” Biochemical Journal, vol. 362, no. 1, pp. 137–147, 2002. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Amer, A. Goldfarb, and E. Fibach, “Flow cytometric measurement of reactive oxygen species production by normal and thalassaemic red blood cells,” European Journal of Haematology, vol. 70, no. 2, pp. 84–90, 2003. View at Publisher · View at Google Scholar · View at Scopus
  21. D. A. Bass, J. W. Parce, and L. R. Dechatelet, “Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation,” Journal of Immunology, vol. 130, no. 4, pp. 1910–1917, 1983.
  22. M. R. Loken, V. O. Shah, K. L. Dattilio, and C. I. Civin, “Flow cytometric analysis of human bone marrow: I. Normal erythroid development,” Blood, vol. 69, no. 1, pp. 255–263, 1987. View at Scopus
  23. Y. Kohgo, K. Ikuta, T. Ohtake, Y. Torimoto, and J. Kato, “Body iron metabolism and pathophysiology of iron overload,” International Journal of Hematology, vol. 88, no. 1, pp. 7–15, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  24. S. Rivella, “Ineffective erythropoiesis and thalassemias,” Current Opinion in Hematology, vol. 16, no. 3, pp. 187–194, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  25. M. Kruszewski, “Labile iron pool: the main determinant of cellular response to oxidative stress,” Mutation Research, vol. 531, no. 1-2, pp. 81–92, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. E. Prus and E. Fibach, “Effect of iron chelators on labile iron and oxidative status of thalassaemic erythroid cells,” Acta Haematologica, vol. 123, no. 1, pp. 14–20, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  27. G. Rechavi and S. Rivella, “Regulation of iron absorption in hemoglobinopathies,” Current Molecular Medicine, vol. 8, no. 7, pp. 646–662, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. E. Prus and E. Fibach, “Effect of iron chelators on labile iron and oxidative status of thalassaemic erythroid cells,” Acta Haematologica, vol. 123, no. 1, pp. 14–20, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  29. A. S. Zhang, F. Canonne-Hergaux, S. Gruenheid, P. Gros, and P. Ponka, “Use of Nramp2-transfected Chinese hamster ovary cells and reticulocytes from mk/mk mice to study iron transport mechanisms,” Experimental Hematology, vol. 36, no. 10, pp. 1227–1235, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  30. J. M. Leimberg, A. M. Konijn, and E. Fibach, “Developing human erythroid cells grown in transferrin-free medium utilize iron originating from extracellular ferritin,” American Journal of Hematology, vol. 73, no. 3, pp. 211–212, 2003. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  31. H. Li, A. C. Rybicki, S. M. Suzuka et al., “Transferrin therapy ameliorates disease in β-thalassemic mice,” Nature Medicine, vol. 16, no. 2, pp. 177–182, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  32. J. M. Leimberg, E. Prus, G. Link, E. Fibach, and A. M. Konijn, “Iron-chelator complexes as iron sources for early developing human erythroid precursors,” Translational Research, vol. 151, no. 2, pp. 88–96, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  33. K. Kalantar-Zadeh, R. A. Rodriguez, and M. H. Humphreys, “Association between serum ferritin and measures of inflammation, nutrition and iron in haemodialysis patients,” Nephrology Dialysis Transplantation, vol. 19, no. 1, pp. 141–149, 2004. View at Publisher · View at Google Scholar · View at Scopus