FK228 Analogues Induce Fetal Hemoglobin in Human Erythroid Progenitors
Figure 2
Known HbF inducers activate γ-globin expression in KU812 cells. (a) Induction of γ-globin transcription by known HbF inducers. Cells were treated for 48 hr with the different drug inducers and γ-globin and β-globin gene transcription were measured by RT-qPCR (see Section 2). The relative mRNA levels were plotted as fold increase. Untreated cells (UT) were used as a control and normalized to one. Data were calculated as the means ± standard error of the mean (SEM); * and **. (b) Shown is a schematic of the dual luciferase reporter construct μLCRβ prRlucγ prFluc which contains a 3.1-kb μLCR cassette linked to a 315-bp human β-globin gene promoter driving the renilla luciferase gene and a 1.4-kb Aγ promoter driving the firefly luciferase gene [33, 39].
