FK228 Analogues Induce Fetal Hemoglobin in Human Erythroid Progenitors
Figure 4
HDAC inhibition assay. The HDAC Fluorescent Activity Assay was used to measure HDAC inhibition in HeLa cells (See Section 2). Compounds and FDL substrate were added to HeLa cells to allow drug activation in vivo then fluorescence levels were quantified on the fluorometer at 440 nm. (a) Trichostatin A (TSA) was used as the control. (b) Inhibition studies were conducted for FK228 showing maximal HDAC inhibition at the 300 nM concentration. (c) Similar studies were performed for JMA33 and JMA26. The data are reported as the means ± SEM.
