Hemin Augments Growth and Hemoglobinization of Erythroid Precursors from Patients with Diamond-Blackfan Anemia

Figure 1

Effect of hemin on free radical generation, apoptosis, and necrosis of erythroid precursors. Mononuclear cells derived from the peripheral blood of normal donors were cultured according to the two-phase liquid culture procedure. After 6 days in phase II, hemin was added at the indicated concentrations for 16 hrs. Cells were harvested and stained with dichlorofluorescein diacetate, phycoerythrin-conjugated Annexin-V, or propidium iodide and analyzed by flow cytometry. The reactive oxygen species (ROS) data are presented as the mean fluorescence channel; apoptosis and necrosis data are presented as the percentage of positive cells. The results (average ± SD N = 4) show a modest dose-dependent stimulation of ROS by hemin but no effect on apoptosis and necrosis.