253989.fig.008a
(a)
253989.fig.008b
(b)
Figure 8: Cell viability testing using LIVE/DEAD assay on trapped MDCK II cells. (a) Initial distribution of MDCK II cells after 50 minutes in an optically patterned hydrogel. The starting cell density was approximately 1 × 105 cells/mL in solution. The dashed circle encloses the area that was gelled. The circular objects that are visible are the cells. (b) Fluorescent image of the same cells from (a), demonstrating approximately 100% viability. Cells lying outside the gel were not taken into account. Live cells fluoresce green, while dead cells fluoresce red. Fluorescent cells appear as bright spots in these images.