253989.fig.009a
(a)
253989.fig.009b
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253989.fig.009c
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Figure 9: Cell viability testing using LIVE/DEAD assay on trapped HeLa cells. (a) Initial distribution of HeLa cells after 45 minutes in an optically patterned hydrogel. The starting cell density was approximately 1 × 106 cells/mL in solution. (b-c) Fluorescent images of the same cells from (a), demonstrating approximately 81% viability. Live cells fluoresce green (b), while dead cells fluoresce red (c). Fluorescent cells appear as bright spots in these images.