Regulation of Hypoxia-Induced Cell Death in Human Tenocytes
Figure 4
Bnip3 and Nix are upregulated by hypoxia in human tenocytes but Bcl2 is not. Primary human tenocytes were exposed to total hypoxia (0.1% O2) for 1, 4, 8, 16, 24, and 48 h. The cells were harvested in cell lysis buffer. (a) Representative Western blot for Bnip3 in tenocytes. (b) Fold change of Bnip3 is presented as mean ± SE (). *Represents versus control. (c) Representative western blot for Nix after hypoxia treatment for 1, 4, 8, 16, 24, and 48 h in tenocytes. (d) Antiapoptotic protein Bcl2 remains at low level after hypoxia treatment. Representative Western blot for Bcl2 after hypoxia treatment for 1, 4, 8, 16, 24, and 48 h in tenocytes.
