Advances in Optical Technologies 
Volume 2008 (2008), Article ID 532368, 10 pages
doi:10.1155/2008/532368
Research Article

A Ratiometric Fluorescence Imaging System for Surgical Guidance

Eduardo H. Moriyama,1,2 Anthony Kim,1 Arjen Bogaards,1 Lothar Lilge,1 and Brian C. Wilson1

 1Division of Biophysics and Bioimaging, Ontario Cancer Institute, University Health Network, 610 University Avenue 7-420, Toronto, ON, M5G 2M9, Canada
2Department of Physics, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada
Received 3 March 2008; Accepted 28 June 2008

Recommended by Stoyan Tanev

Abstract

A 3-chip CCD imaging system has been developed for quantitative in vivo fluorescence imaging. This incorporates a ratiometric algorithm to correct for the effects of tissue optical absorption and scattering, imaging “geometry” and tissue autofluorescence background. The performance was characterized, and the algorithm was validated in tissue-simulating optical phantoms for quantitative measurement of the fluorescent molecule protoporphyrin IX (PpIX). The technical feasibility to use this system for fluorescence-guided surgical resection of malignant brain tumor tissue was assessed in an animal model in which PpIX was induced exogenously in the tumor cells by systemic administration of aminolevulinic acid (ALA).