A Rapid, Isocratic HPLC Method for Determination of Insulin and Its Degradation Product

Table 3

Data of accuracy experiments.

Conc. mg/mL	Insulin area	IS area	AR*	Calc. conc.	Average calc. conc.	Standard deviation	RSD %	Re. error %
0.17025	28.45996	9.47664	3.00317	0.17275	0.17158	0.00158	0.92	0.78
	27.98159	9.34673	2.99373	0.17221
	25.80710	8.74393	2.95143	0.16978
0.06810	10.95933	9.13004	1.20036	0.06923	0.06906	0.00045	0.65	1.41
	11.27432	9.36966	1.20328	0.06940
	11.44450	9.62872	1.18858	0.06855
0.01703	2.85957	9.81390	0.29138	0.01703	0.01672	0.00029	1.73	−1.79
	2.74505	9.63243	0.28498	0.01667
	3.02176	10.7402	0.28135	0.01646

∗AR: area ratio.