]>Methods to Evaluate the Effect of Ethanol on the Folate Analogue: Fluorescein Methotrexate Uptake in Human Proximal Tubular Cells : Figure 5
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Figure 5: Fluoroskan studies of FL-MTX (10  𝜇 M) uptake by HPT cells in 24 well plates treated with ethanol (500 mg/dL), ET-B receptor antagonist (RES-701-1) (10  𝜇 M), and PKC selective inhibitor (BIM) (2.5  𝜇 M). Inhibition of FL-MTX uptake is reversed by the addition of RES-701-1 or BIM. Data are expressed as mean ± SEM ( 𝑛 = 5 ).