Review Article
Key Role of Human ABC Transporter ABCG2 in Photodynamic Therapy and Photodynamic Diagnosis
Table 1
PCR primers to quantitatively measure the mRNA levels of PEPT1, PEPT2, ALAS1, ALAD, HMBS, UROS, UROD, ABCB6 CPOX, PPOX, ABCG2, FECH, HO-1, and HIF1alpha.
| Gene | F/R | Primer sequence | Position | Tm |
| PEPT1 | Forward | CCCTGAAGTGAAGGTGTTTGAAGATA | 1772–1797 | 60.6 | NM_005073 | Reverse | GAATTGGCCCCTGACATGAA | 2168–2188 | 59.3 | PEPT2 | Forward | CTACCACAATATGCCCTGGTTACA | 1894–1917 | 58.8 | NM_001145998 | Reverse | GCCACTGAACTGTGCCACAA | 2045–2064 | 59.3 | ALAS1 | Forward | GGATTCGAAACAGCCGAGTG | 1371–1390 | 59.3 | NM 000688 | Reverse | GAAGGTGATTGCTCCAAACTCAT | 1542–1564 | 58.3 | ALAD | Forward | CCTCGGTTCCAACCAACTGAT | 157–177 | 59.8 | NM_000031 | Reverse | GATAGGCTGTATGTCATCAGGAACA | 319–343 | 58.2 | HMBS | Forward | CAAGGACCAGGACATCTTGGAT | 835–856 | 58.9 | NM_000190 | Reverse | CCAGACTCCTCCAGTCAGGTACA | 984–1006 | 59.2 | UROS | Forward | TCAGCACTGCCTCTTCTATTTCC | 668–691 | 58.7 | NM_000375 | Reverse | CTGGGTGTGCAACTGTCTGATAC | 761–789 | 58.3 | UROD | Forward | CGGGAGTGTGTGGGGAA | 982–998 | 57.2 | NM_000374 | Reverse | AAGCAGACGTGAGTGTTTATGCA | 1178–1200 | 58.6 | ABCB6 | Forward | CAGAAGGGCCGTATTGAGTTTG | 2033–2054 | 59.6 | NM_005689 | Reverse | ATTGTCGGCGATGGTGTCA | 2308–2326 | 59.5 | CPOX | Forward | GGCGGAGATGTTGCCTAAGAC | 401–421 | 59.7 | NM_000097 | Reverse | AATGCTCACCCCAGCCTTTT | 709–728 | 59.5 | PPOX | Forward | CAGGAGTCCTGGGAATCGTGTA | 1251–1272 | 59.9 | NM_000309 | Reverse | TGCCTAGCTGACTCTAGTTTTTGC | 1509–1533 | 58.1 | FECH | Forward | GGAAATCCATTGTTCTCTAAGGC | 1252–1274 | 57.0 | NM_001012515 | Reverse | CTAAATAACACCCTCTCCACATCG | 1462–1485 | 57.8 | ABCG2 | Forward | CTAAGCAGGGACGAACAATCATC | 1188–1210 | 58.8 | NM_004827 | Reverse | TCCTGCTTGGAAGGCTCTATG | 1447–1467 | 58.2 | HIF-1alpha | Forward | GGCGCGAACGACAAGAA | 420–436 | 58.0 | NM 001530 | Reverse | CAAAACCATCCAAGGCTTTCA | 682–702 | 58.7 | HO-1 | Forward | GCTCAAAAAGATTGCCCAGAA | 518–538 | 58.1 | NM_002133 | Reverse | TCACATGGCATAAAGCCCTACA | 926–947 | 59.1 |
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PEPT1: oligopeptide transporter 1; PEPT2: oligopeptide transporter 2; ALAS1: delta-aminolevulinate synthase 1; ALAD, delta-aminolevulinate dehydratase; HMBS: hydroxymethylbilane synthase; UROS: uroporphyrinogen III synthase; UROD: uroporphyrinogen decarboxylase; ABCB6: ABC transporter B6; CPOX: coproporphyrinogen oxidase; PPOX: protoporphyrinogen oxidase; FECH: ferrochelatase; ABCG2: ABC transporter G2 (BCRP); HIF-1alpha: hypoxia inducible factor-1 alpha subunit; HO-1: heme oxygenase-1. F/R: forward or reverse primers; Tm: melting temperature.
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