The Role of Tetrahydrobiopterin and Dihydrobiopterin in Ischemia/Reperfusion Injury When Given at Reperfusion

Table 1

The effects of BH₄ or BH₂ on NO release from nonischemic rat aortic segments.

Basal/Treatments	NO release; (picomoles/mg tissue)	Sample No.
Basal	2.28 ± 0.25	𝑛=17
Ach (5 𝜇M)	5.38±0.70^∗∗	𝑛=20
BH4 (1 𝜇M)	2.74 ± 0.16	𝑛=14
BH4 (10 𝜇M)	4.45 ± 0.43*	𝑛=21
BH4 (20 𝜇M)	4.88±0.50^∗∗	𝑛=24
BH4 (40 𝜇M)	5.01±0.61^∗∗	𝑛=21
BH2 (50 𝜇M)	0.87±0.33^##	𝑛=21
BH2 (100 𝜇M)	0.86±0.23^##	𝑛=25
BH2 (200 𝜇M)	0.78±0.33^##	𝑛=14
Ach (5 𝜇M)+L-NAME (800 𝜇M)	−0.59±0.38	𝑛=5
BH4 (10 𝜇M)+L-NAME (800 𝜇M)	−1.14±0.48	𝑛=8
BH2 (100 𝜇M)+L-NAME (800 𝜇M)	−1.70±0.34	𝑛=8

^∗𝑃<.05,^∗∗𝑃<.01 compared to basal NO release; ^##𝑃<.01 compared to the effects of BH₄ on NO release.