304317.fig.001
Figure 1: Expression of differentiation-specific protein involucrin of NHEK after treatment with 10 μg/mL St. John’s Wort polysaccharide fractions for nine days. Protein amounts were determined by semiquantitative dot blot technique. Obtained values with were normalized to untreated NHEK (—: untreated NHEK = 100%). To induce the differentiation, the serum-starved MCDB 153 complete media were supplemented with 13 μg/mL A23187 and 2 mM Ca2+ (positive control).