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Advances in Pharmacological Sciences
Volume 2013 (2013), Article ID 512931, 7 pages
http://dx.doi.org/10.1155/2013/512931
Research Article

Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis

1Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Immunology, Qazvin University of Medical Sciences, Qazvin, Iran
4Pharmacognosy and Pharmaceutics, Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
5Department of Food and Drug Control, School of Pharmacy, Tehran University of Medical Science, Tehran, Iran
6Plant Breeding and Biotechnology Department, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Received 28 August 2012; Revised 7 December 2012; Accepted 17 December 2012

Academic Editor: Robert Gogal

Copyright © 2013 Fatemeh Zare Shahneh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Echinophora platyloba DC plant (Khousharizeh) is one of the indigenous medicinal plants which is used as a food seasoning and medicine in Iran. The objective of this study was to examine the in vitro cytotoxic activity and the mechanism of cell death of crude methanolic extracts prepared from Echinophora platyloba DC, on mouse fibrosarcoma cell line (WEHI-164). Cytotoxicity and viability of methanolic extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Cell death ELISA was employed to quantify the nucleosome production result from nuclear DNA fragmentation during apoptosis and determine whether the mechanism involves induction of apoptosis or necrosis. The cell death was identified as apoptosis using terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP nick end labeling (TUNEL) assay. Our results demonstrated that the extract decreased cell viability, suppressed cell proliferation, and induced cell death in a time- and dose-dependent manner in WEHI-164 cells (IC50 = 196.673 ± 12.4 μg/mL) when compared with a chemotherapeutic anticancer drug, Toxol. Observation proved that apoptosis was the major mechanism of cell death. So the Echinophora platyloba DC extract was found to time- and dose-dependently inhibit the proliferation of fibrosarcoma cell possibly via an apoptosis-dependent pathway.