Table 1: The comparative effects of in vitro incubation with two calcineurin inhibitors (CNI), cyclosporine (CsA, 1.0 mg/mL), or tacrolimus (Tac, 0.1 mg/mL) for 30 min duration on acetylcholine (ACh)-induced vasodilator responses in phenylephrine (PE, 1 µM)-constricted thoracic and abdominal rat aortic rings with intact endothelium.

RegionIncubation conditionACh-DRC: Mean ± SEM values
IC50

Thoracic aortaControl68 ± 16.4 nM98% ± 1.3%
CsA (1.0 mg/mL)9.5 ± 2.55 µM**10% ± 2.2%**
Tac (0.1 mg/mL)222 ± 60.2 nM*97% ± 2.7%

Abdominal aortaControl50 ± 17.8 nM 100% ± 0.0%
CsA (1.0 mg/mL)7.0 ± 3.14 µM**58% ± 4.94%**
Tac (0.1 mg/mL)215 ± 58.6 nM*80% ± 5.79%*

and ** compared to respective control. Each value is mean ± SEM of several experiments performed using rat aortic rings isolated from 6 rats for each condition of incubation.
Note: vehicle control studies with castor oil alone yielded closely similar values like control data performed in the presence of with Kreb’s buffer alone.