Development of Mass Spectrometry Selected Reaction Monitoring Method for Quantitation and Pharmacokinetic Study of Stepharine in Rabbit Plasma

Table 1

Stepharine extraction recovery from rabbit plasma using acetonitrile and methanol.

Extracting organic solvent	Concentration of stepharine in preextracted plasma, 𝜇g/mL	Postextraction recovery, % ± RSD
Acetonitrile	3	85.4±5.2
	50	87.8±3.3
	100	92.2±3.7
Methanol	3	67.1±4.7
	50	66.3±6.1
	100	69.1±4.9

∗The results are averaged on five replicates (𝑛=5).