Development of Mass Spectrometry Selected Reaction Monitoring Method for Quantitation and Pharmacokinetic Study of Stepharine in Rabbit Plasma

Table 2

Concentration of stepharine in rabbit plasma after intramuscular administration*.

Time after administration, minutes	Animal internal ID
	ST01	ST02	ST03	ST04	ST05	ST06
	Measured mean concentration of stephaglabrin in plasma, ng/mL
0	0	0	0	0	0	0
15	8.1±0.6	7.9±0.2	6.8±0.4	5.7±0.2	6.3±0.2	7.0±0.3
30	9.4±0.5	16.5±0.8	14.8±0.2	7.3±0.3	12.7±0.3	13.9±0.2
60	10.0±0.3	29.3±2.2	19.5±0.6	9.5±0.5	18.5±0.2	20.4±0.2
90	13.9±0.9	33.9±2.8	20.4±1.7	14.2±0.2	21.2±0.2	25.8±0.1
120	5.8±0.1	10.4±0.5	6.5±0.1	9.7±0.2	5.1±0.3	7.6±0.09
180	3.8±0.1	7.8±0.3	n/a	2.8±0.2	2.9±0.07	3.3±0.08
480	1.8±0.02	1.2±0.02	n/a	0.7±0.01	1.4±0.08	1.2±0.08
720	1.5±0.02	1.3±0.01	n/a	0.6±0.01	0.9±0.04	0.7±0.02
1440	0	0	n/a	0	0	0

∗Measurements were made up by using calibration curve and averaged on five replicates.