Simple and Robust Analysis of Cefuroxime in Human Plasma by LC-MS/MS: Application to a Bioequivalence Study

Table 2

Summary of the stability of cefuroxime in human plasma on different conditions (𝑛=3).

Stability conditions	Concentration (𝜇g/mL)	Calculated concentration
		Mean ± SD (𝜇g/mL)	Accuracy%	RSD%
Short-term (6 h, 25°C)	0.0842	0.0799±0.0022	94.97	2.75
	1.68	1.70±0.025	100.8	1.47
	16.8	16.4±0.06	97.63	0.40
Long-term (145 days, −20°C)	0.105	0.103±0.004	98.26	4.22
	1.68	1.73±0.038	103.0	2.25
	16.8	16.77±0.40	99.94	2.36
Autosampler (20 h, 4°C)	0.0842	0.0823±0.0030	97.76	3.89
	1.68	1.71±0.025	101.8	1.47
	16.8	16.6±0.27	98.79	1.63
Three freeze-thaw cycles (from 25°C to −20°C)	0.0842	0.0837±0.0048	99.46	5.76
	1.68	1.72±0.032	102.1	1.84
	16.8	16.6±0.36	98.65	2.15