Spirulina platensis Lacks Antitumor Effect against Solid Ehrlich Carcinoma in Female Mice

Figure 3

Histopathological examination of Ehrlich ascites carcinoma (EAC) solid tumor. Representative sections were obtained from (a) untreated EAC-bearing mice (arrows: infiltration of subcutaneous tissue with tumor cells), (b) untreated EAC-bearing mice (arrows: newly formed blood capillaries; arrowheads: leukocyte infiltration), (c) EAC tumor-bearing mice treated with 5-fluorouracil 20 mg/kg (arrowheads: extensive necrosis; arrow: fibrosis; M: skeletal muscles), (d) EAC tumor-bearing mice treated with 5-fluorouracil 20 mg/kg (irregular arrow: destructed blood vessels and hemorrhage), (e) EAC tumor-bearing mice treated with spirulina 200 mg/kg (arrows: extensive infiltration of the subcutaneous tissue with tumor cells), (f) EAC tumor-bearing mice treated with spirulina 200 mg/kg (arrows: tumor cells with cellular anaplasia and anisocytosis), (g) EAC tumor-bearing mice treated with spirulina 800 mg/kg (arrows: moderate infiltration of the tumor cells; arrowhead: numerous leukocyte infiltration), (h) EAC tumor-bearing mice treated with spirulina 800 mg/kg (arrows: huge numbers of tumor cells infiltrating the skeletal muscles), (i) EAC tumor-bearing mice treated with 5-fluorouracil 20 mg/kg plus spirulina 200 mg/kg (arrows: less infiltration with tumor cells; N: extensive necrosis and fibrosis), and (j) EAC tumor-bearing mice treated with 5-fluorouracil 20 mg/kg plus spirulina 200 mg/kg (arrows: islets of viable tumor cells; N: extensive necrosis). Sections were stained with HE dyes (scale bar = 50 μM).

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