Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

Figure 1

Effect of raw extract and fractions from E. tereticornis on insulin-resistant C2C12 and HepG2 cells. Glucose uptake in insulin-resistant C2C12 cells (a) and glucose production in insulin-resistant HepG2 cells (b). Cells were treated with different concentrations of the fractions or raw extract and glucose was measured in cultured supernatant after 4 h of treatment by the glucose oxidase technique. Eu, crude methanol extract; Ins (100 nM insulin); Control R, insulin-resistant C2C12 myotubes as controls; Ins 100 R, insulin-resistant C2C12 myotubes treated with 100 nM insulin; Metf, metformin 1 mM positive control. Bar values correspond to the arithmetic mean of glucose concentration for each treatment/control glucose concentration, (C2C12) and (HepG2). 100 nM versus control. versus control R. , Student t-test. Error bars represent SEM.

(a)

(b)