Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae

Table 1

Method validation parameters for estimation of luteolin and apigenin.

Parameters	Luteolin	Apigenin
Wavelength, nm	366	366
Linearity range, ng/band	200–1000	50–250
Regression equation
Correlation coefficient	0.9942	0.9917
Limit of detection, ng/band	42.6	7.97
Limit of quantification, ng/band	129.08	24.155
Specificity	Specific	Specific