Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts
Table 2
Myocardial tissue parameters.
Normoxia
Hypoxia
Reoxygenation
Aspartate aminotransferase (AST)
AST: U × L−1 × g−1 heart tissue (; mean ± SD)
2.934 ± 1.451 (100%)
3.249 ± 1.119 (110.7%)
4.105 ± 1.917 (139.9%)
N versus R
Curc. 0.25 µM
3.382 ± 2.149 (115.3%)
2.397 ± 0.901 (81.7%)
R versus
Curc. 0.5 µM
3.671 ± 1.446 (125.1%)
3.192 ± 1.516 (108.8%)
TBARS, expressed as malondialdehyde (MDA)
MDA: nmol × g−1 heart tissue (; mean ± SD; N versus R and H versus )
8.33 ± 5.30 (100%)
9.36 ± 6.24 (112.4%)
19.37 ± 8.31 (232.5%; N versus R )
Curc. 0.25 µM
8.86 ± 0.48 (106.4%)
12.46 ± 10.30 (149.6%)
Curc. 0.5 µM
5.88 ± 5.85 (70.6%)
12.52 ± 6.85 (150.3%)
H versus ()
The activity of AST was measured in the perfusate as U × L−1 and then correlated to the weight of the heart (= g heart tissue). TBARS, thiobarbituric acid reactive substances; N, normoxia; H, hypoxia; R, reoxygenation; , reoxygenation with curcumin (curc.) 0.25 µM; , hypoxia with 0.5 µM curc.
