Research Article
Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29
Table 1
Retention time of 18 standard phenolic acids and flavonoids, and results of HPLC analysis of Origanum majorana extracts with different solvents.
| | Standards | RT (min) | Petroleum ether | Dichloromethane | Ethyl acetate | Methanol | Depleted aqueous | Aqueous |
| | Chlorogenic acid | 9.03 | − | − | − | − | − | + | | Catechin | 9.13 | − | − | − | − | + | + | | 3,4-Dihydropyran | 9.50 | + | − | − | − | + | − | | 2-(4-Hydroxy phenyl) ethanol | 10.20 | + | − | + | + | + | + | | Vanillic acid | 10.33 | − | − | + | + | − | + | | 4-Hydroxybenzoic acid | 10.42 | − | − | + | − | − | + | | Syringic acid | 10.51 | + | − | + | − | + | + | | Caffeic acid | 11.01 | + | − | + | − | + | + | | Vanillin | 11.25 | + | − | + | − | + | + | | p-Coumaric acid | 12.63 | − | − | − | − | + | + | | trans-Ferulic acid | 13.25 | + | + | + | + | + | + | | Rutin | 15.22 | + | + | − | + | − | + | | o-Coumaric acid | 15.27 | + | + | − | + | + | + | | Luteolin | 16.54 | + | + | + | − | − | + | | Cinnamic acid | 16.62 | + | + | + | − | − | + | | Apigenin | 17.36 | − | + | − | − | − | − | | Quercetin | 17.82 | + | + | − | − | − | − | | trans-Chalcone | 20.45 | + | − | − | − | − | − |
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“+” sign indicates the presence of phenolic compounds; “−” sign indicates the absence of phenolic compounds.
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