In Vitro Mechanism of Action of Acanthospermum hispidum in Trypanosoma brucei

Table 1

Antitrypanosomal activities of fractions.

Fractions	Mean EC50 (μg/mL) ± SEM		SI
	T. b. brucei	RAW 264.7
AH-HEX	2.4 ± 0.06	84.3 ± 1.1	35.1
AH-DCM	2.2 ± 0.06	68.9 ± 0.9	38.3
AH-EA	1.0 ± 0.06	92.8 ± 1.0	92.8
AH-AQ	2.0 ± 0.10	87.5 ± 1.0	43.8
DA	0.6 ± 0.09	74.1 ± 1.1	123.5

SI (selectivity index) was estimated as the ratio of the EC₅₀ value in RAW 264.7 cell lines to the EC₅₀ value in T. b. brucei. High SI values denote greater selectivity towards T. b. brucei in the presence of RAW 264.7 cell lines. Standard errors of the mean (SEM) were calculated from quadruplicate sets of EC₅₀ values. Hexane fraction = AH-HEX, dichloromethane fraction = AH-DCM, ethyl-acetate fraction = AH-EA, aqueous fraction = AH-AQ, DA = Diminazene aceturate.