Research Article
Enhancement of Liver Targetability through Statistical Optimization and Surface Modification of Biodegradable Nanocapsules Loaded with Lamivudine
Table 2
Combinations of the formulation factors according to the selected factorial design and the observed results
of characterization studies of LAM-ACNs.
| | Formulation code | Factor level | EEa (%) | Particle size (nm) | Zeta potential (mV) | kc (h−1) | | A | B | C |
| | F1 | −1 | −1 | −1 | 21.59 ± 3.18 | 305.2 ± 3.4 | −24.6 ± 1.5 | 0.243 ± 0.02 | | F2 | −1 | 0 | −1 | 32.14 ± 2.54 | 289.3 ± 2.5 | −26.9 ± 1.7 | 0.224 ± 0.05 | | F3 | −1 | +1 | −1 | 28.42 ± 2.67 | 274.3 ± 1.9 | −25.7 ± 0.9 | 0.206 ± 0.03 | | F4 | −1 | −1 | 0 | 32.68 ± 3.22 | 302.1 ± 5.2 | −26.3 ± 2.1 | 0.218 ± 0.03 | | F5 | −1 | 0 | 0 | 48.29 ± 1.96 | 286.5 ± 4.2 | −22.9 ± 2.3 | 0.186 ± 0.04 | | F6 | −1 | +1 | 0 | 42.06 ± 4.06 | 272.3 ± 2.6 | −25.3 ± 1.4 | 0.165 ± 0.02 | | F7 | −1 | −1 | +1 | 44.52 ± 3.28 | 268.3 ± 2.1 | −24.3 ± 1.8 | 0.196 ± 0.06 | | F8 | −1 | 0 | +1 | 54.38 ± 4.25 | 242.5 ± 3.2 | −24.9 ± 0.8 | 0.174 ± 0.03 | | F9 | −1 | +1 | +1 | 52.29 ± 2.98 | 231.3 ± 1.8 | −26.2 ± 1.5 | 0.147 ± 0.04 | | F10 | 0 | −1 | −1 | 38.96 ± 2.17 | 324.8 ± 2.7 | −25.6 ± 2.3 | 0.206 ± 0.03 | | F11 | 0 | 0 | −1 | 43.58 ± 3.26 | 318.2 ± 5.7 | −23.7 ± 1.7 | 0.197 ± 0.01 | | F12 | 0 | +1 | −1 | 42.19 ± 3.09 | 304.3 ± 3.1 | −24.3 ± 1.1 | 0.165 ± 0.03 | | F13 | 0 | −1 | 0 | 46.36 ± 4.21 | 322.6 ± 3.6 | −22.9 ± 3.1 | 0.186 ± 0.02 | | F14 | 0 | 0 | 0 | 57.47 ± 3.84 | 313.9 ± 2.3 | −24.1 ± 2.4 | 0.178 ± 0.06 | | F14 | 0 | 0 | 0 | 56.39 ± 2.61 | 310.6 ± 1.4 | −25.3 ± 1.9 | 0.169 ± 0.04 | | F15 | 0 | +1 | 0 | 52.62 ± 2.46 | 299.6 ± 2.8 | −23.9 ± 1.8 | 0.154 ± 0.02 | | F16 | 0 | −1 | +1 | 59.72 ± 4.22 | 315.9 ± 3.5 | −24.9 ± 2.1 | 0.143 ± 0.03 | | F17 | 0 | 0 | +1 | 69.84 ± 1.96 | 302.5 ± 2.2 | −25.4 ± 1.3 | 0.126 ± 0.05 | | F18 | 0 | +1 | +1 | 66.92 ± 2.05 | 294.1 ± 4.7 | −23.7 ± 3.2 | 0.112 ± 0.02 | | F19 | +1 | −1 | −1 | 36.85 ± 2.31 | 341.2 ± 4.3 | −28.1 ± 2.6 | 0.175 ± 0.04 | | F20 | +1 | 0 | −1 | 42.52 ± 1.87 | 320.5 ± 3.9 | −27.3 ± 2.9 | 0.162 ± 0.03 | | F21 | +1 | +1 | −1 | 40.36 ± 3.04 | 311.6 ± 1.5 | −25.2 ± 1.6 | 0.148 ± 0.04 | | F22 | +1 | −1 | 0 | 52.39 ± 2.12 | 336.1 ± 1.9 | −26.7 ± 1.2 | 0.159 ± 0.05 | | F23 | +1 | 0 | 0 | 59.86 ± 3.28 | 323.5 ± 2.4 | −24.6 ± 1.9 | 0.153 ± 0.02 | | F24 | +1 | +1 | 0 | 53.58 ± 2.31 | 308.1 ± 1.5 | −22.7 ± 0.7 | 0.138 ± 0.02 | | F25 | +1 | −1 | +1 | 63.93 ± 1.86 | 322.5 ± 3.1 | −26.2 ± 2.2 | 0.121 ± 0.04 | | F26 | +1 | 0 | +1 | 73.41 ± 2.13 | 302.4 ± 2.5 | −26.7 ± 2.1 | 0.108 ± 0.03 | | F27 | +1 | +1 | +1 | 69.35 ± 2.54 | 286.9 ± 2.3 | −25.8 ± 2.7 | 0.094 ± 0.02 |
|
|
The results were expressed as average ± standard deviation for n = 3. |
