Advances in Pharmacological and Pharmaceutical Sciences

Research Article

Enhancement of Liver Targetability through Statistical Optimization and Surface Modification of Biodegradable Nanocapsules Loaded with Lamivudine

Table 3

Results of ANOVA for the quadratic model for three selected response variables.


S. no.	Response	Source	SS^a	Df^b	MSS^c	Fvalue	value	Inference^d

1	EE^h (%)	Model	4617.96	9	513.11	174.90	<0.0001	Significant
		A^e	1025.74	1	1025.74	349.63	<0.0001	Significant
		B^f	143.31	1	143.31	48.85	<0.0001	Significant
		C^g	2881.67	1	2881.67	982.24	<0.0001	Significant
		AB	16.01	1	16.01	5.46	0.0313	Significant
		AC	26.76	1	26.76	9.12	0.0074	Significant
		BC	3.88	1	3.88	1.32	0.2654	Not significant
		A²	214.65	1	214.65	73.17	<0.0001	Significant
		B²	264.47	1	264.47	90.15	<0.0001	Significant
		C²	1.08	1	1.08	0.37	0.5522	Not significant
		Residual	52.81	18	2.93
		Lack of fit	52.22	17	3.07	5.27	0.3315	Not significant

2	Particle size (nm)	Model	16802.45	9	1866.94	38.11	<0.0001	Significant
		A	8064.50	1	8064.50	164.60	<0.0001	Significant
		B	3464.58	1	3464.58	74.43	<0.0001	Significant
		C	2762.72	1	2762.72	56.39	<0.0001	Significant
		AB	1.69	1	1.69	0.034	0.8548	Not significant
		AC	354.25	1	354.25	7.23	0.0150	Significant
		BC	14.96	1	14.96	0.31	0.5873	Not significant
		A²	1312.04	1	1312.04	26.78	<0.0001	Significant
		B²	19.61	1	19.61	0.40	0.5349	Not significant
		C²	533.27	1	533.27	10.88	0.0040	Significant
		Residual	881.88	18	48.99
		Lack of fit	876.43	17	51.55	9.47	0.2508	Not significant

3	kⁱ (h⁻¹)	Model	0.035	9	3.84 × 10⁻³	63.80	<0.0001	Significant
		A	0.014	1	0.014	231.81	<0.0001	Significant
		B	5.62 × 10⁻³	1	5.62 × 10⁻³	93.39	<0.0001	Significant
		C	0.014	1	0.014	235.53	<0.0001	Significant
		AB	3.41 × 10⁻⁴	1	3.41 × 10⁻⁴	5.67	0.0285	Significant
		AC	3.00 × 10⁻⁶	1	3.00 × 10⁻⁶	0.05	0.8258	Not significant
		BC	3.33 × 10⁻⁷	1	3.33 × 10⁻⁷	0.0055	0.9415	Not significant
		A²	1.36 × 10⁻⁴	1	1.36 × 10⁻⁴	2.27	0.1493	Not significant
		B²	3.10 × 10⁻⁵	1	3.10 × 10⁻⁵	0.52	0.4819	Not significant
		C²	3.16 × 10⁻⁴	1	3.16 × 10⁻⁴	5.25	0.0342	Significant
		Residual	1.08 × 10⁻³	18	6.13 × 10⁻⁵
		Lack of fit	1.04 × 10⁻³	17	4.05 × 10⁻⁵	1.51	0.5724	Not significant

Note. ^aSum of squares; ^bdegrees of freedom; ^cmean sum of squares; ^d value less than 0.05 indicates model terms are significant; ^epolymer concentration (%w/w); ^fsurfactant concentration in secondary emulsion (%w/v); ^gconcentration of glycerol in external phase (%v/v); ^hentrapment efficiency; ⁱrelease rate constant.