One-Step Formation of Chondrocytes through Direct Reprogramming via Polysaccharide-Based Gene Delivery
Figure 5
Gene transfection efficiency analyses. Notes. (a) Enzyme-linked immunosorbent assay (ELISA): Lane 1: Blank (cells treated with pmPPY only), Lane 2: Free plasmid Sox9, Lane 3: PEI/pSox9 (2000-folds of dilution), Lane 4: Lipofectamine 2000 /pSox9, Lane 5-8: pSox9/pmPPY nanoparticles in ratios of 15:1, 30:1, 50:1, and 100:1;(b) RT-PCR, Lane 1: Blank (cells treated with pmPPY only), Lane 2: Free plasmid Sox9, Lane 3: PEI/pSox9 (2000-folds of dilution), Lane 4: Lipofectamine 2000 /pSox9, Lane 5-8: pSox9/pmPPY nanoparticles in ratios of 15:1, 30:1, 50:1, and 100:1 (c) Western Blot, Lane (1-4): Blank (cells treated with pmPPY only), Free plasmid Sox9, Lipofectamine 2000 /pSox9, PEI (2000-fold dilution)/pSox9, Lane 5-8, pmPPY-pSox9 nanoparticles prepared at different weight ratios 15:1, 30:1, 50:1, and 100:1, from left to right; and a quantitative analysis of the relative expression levels of Sox9 (mean±standard deviation of measurements from three replicates) P<0.05, P<0.01.