Table 1: Oligonucleotides used in this study.

Primer Sequence (5′–3′) Description RT1.for/rev AATATGCCCTGGAGATAGCG/AGACGACACTTTCCGATACG Used in quantitative PCR for plasmid copy number determination RT2.for/rev ATGCACGAATGCCTACTCTC/GGGCAAAGGAAGGCTTAAAC 16S-Pt.for/rev TCGTCCTTCCAGGATTACAG/CCGGCTTTGTAAATCTCCAG RC1.rev1/2 TCGGATTTAACAGGGCGTCTA/CCTGAACTGCCTGTTATCAAT ORF1 inner/outer 5′RACE RC2.rev1/2 CAATAGGGTGGAGATGTTATAGC/TCAAAGCCCAGCCATTAGATTGC ORF2 inner/outer 5′RACE RC3.rev1/2 TTACCTGACCCTTTATTTCC/TCCAGGGCGTTTAAGTAAATG ORF3 inner/outer 5′RACE RC4.rev1/2 TTTCCCTGATATTCCCTTATCC/ACATCTCTTGGTATGCCTTTC ORF4 inner/outer 5′RACE RC5.rev1/2 TCGGAGTAGAATTTACCTGTAG/GGATAAGGATTACCTCTGTTAG ORF5 inner/outer 5′RACE RC6.rev1/2 GCATTTAGGACAGGTCGCATAC/ACTCACTACCACTTACATAC ORF6 inner/outer 5′RACE