Table 1: Assignments of m/z values detected in the negative ion mode MALDI-TOF mass spectra and PSD analyses of the P. furiosus total lipid extract to various lipid components. Lipids are indicated as abbreviations.
Observed M–H− signals (m/z)
Assignments
Calculated M–H− (m/z)
1784.6
(Hexose-P)2-T
1784.3
1741.5
N-Acetyl-hexose-hexose-P-Tcyclic
1741.4
1701.9
Hexose2-P-T cyclic
1702.4
1543.5
BPG plus Na+
1542.3
1521.3
BPG
1520.3
976.2
DGD
975.8
934.8
N-Acetyl-hexose-P-D
934.7
922.6
uns-N-Acetyl-hexose-P-D
922.6
893.8
PI
893.7
881.6
unsPI
881.5
867.2
PGP minus H2O
868.6
814.4
MGD
813.7
805.7
PG
805.7
787.5
PG minus H2O
787.7
731.8
PA
731.6
241.3
Hexose-P minus H2O
242.0
Abbreviations—D: diether or archaeol; T: tetraether or caldarchaeol; Tcyclic: caldarchaeol with cyclopentane rings; P: phosphate group; uns: unsaturated branched-chains, 3 double bonds for chain; N-acetyl-hexose: N-acetylglucosamine; PI: diphytanylglycerol analogue of phosphatidylinositol; BPG: diphytanylglycerol analogue of bisphosphatidylglycerol (or ether lipid cardiolipin); PG: diphytanylglycerol analogue of phosphatidylglycerol; PGP: diphytanylglycerol analogue of phosphatidylglycerol-phosphate; PA: diphytanylglycerol analogue of phosphatidic acid; MGD: monoglycosyl-diphytanylglycerol; DGD: diglycosyl-diphytanylglycerol.