Mutational and Bioinformatic Analysis of Haloarchaeal Lipobox-Containing Proteins
Figure 2
Hfx. volcanii proteins Hvo_B0139 and Hvo_1242 are Tat substrates that require the lipobox cysteine for processing but not for anchoring to the cytoplasmic membrane. Western blot analyses of the wild-type (RR), twin lysine replacement mutants (KK), and cysteine to serine replacement mutants (C21S and C26S for Hvo_B0139, and Hvo_1242, resp.). All proteins expressed had C-terminal Myc-tags and were detected using anti-Myc antibodies. Comparable amounts of protein were loaded in each lane. The migration of molecular weight standards is indicated on the right. Predicted positions of precursor (p) and mature (m) proteins are indicated.
