Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes

Figure 1

Growth analysis of M. mazei Δhpt versus wild type in the presence of 8-ADP. Two different media were tested, (a) standard minimal medium [18] and (b) minimal medium with significantly reduced yeast extract (0.5 g/L). M. mazei strains were grown in 50 mL of the respective medium complemented with 150 mM methanol as carbon source under a gas atmosphere of N₂/CO₂ (80 : 20). Strains were incubated without additives (open symbols) or in the presence of 20 𝜇g/mL 8-ADP (closed symbols). Squares: wild type; triangles: M. mazei Δhpt. Standard deviations of three replicates for each strain are indicated.

(a)

(b)