Research Article
Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes
Table 1
Strains and plasmids used in this study.
| | Strain or plasmid | Genotype or description | Source or reference |
| | Strains | | |
| | Methanosarcina mazei strain Gö1 | Wild type | DSM No. 3647 | | M. mazei hpt | M. mazei, with hpt deletion | This study | | M. mazei sRNA154 | M. mazei, with sRNA154 deletion | This study | | E. coli DH5α | general cloning strain | Stratagene, La Jolla, US | | E. coli DH5α/λpir | general cloning strain | [16] |
| | Plasmids | | |
| | pMCl210 | general cloning vector | [17] | | pBSK+ | general cloning vector | Stratagene, La Jolla, US | | pDRIVE | general cloning vector | Qiagen, Hilden, Germany | | pRS207 | pac-resistance cassette in pSL1180 | [8] | | pRS269 | pmcr of M. voltae in pDRIVE | This study | | pRS283 | M. mazei hpt deletion construct in pBSK+ | This study | | pRS311 | pBSK+ plus M. mazei hpt gene | This study | | pRS320 | pRS311 with pmcr upstream of hpt | This study | | pRS345 | pRS311 with pac-resistance cassette | This study | | pRS606 | pMCL210 with 930 bp of sRNA154 upstream region | This study | | pRS631 | pMCl210 plus sRNA154 deletion construct | This study | | pRS632 | 154 construct (pRS631) inserted into the ApaI site of pRS345 | This study |
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