Regulatory Multidimensionality of Gas Vesicle Biogenesis in Halobacterium salinarum NRC-1

Figure 2

The contribution of free gas vesicles to light scattering at 600 nm. Gas vesicles were purified as described in Materials and Methods. To replicate the concentration of viable cells at points a and b (Figure 1) cells grown to OD₆₀₀=0.4 were concentrated to OD₆₀₀=1.0 and OD₆₀₀=0.5, respectively. These OD₆₀₀ represent a baseline of scattering form lightly-vacuolated/non-lysed cells at equivalent CFUs to points a and b in Figure 1. Purified gas vesicles were added to concentrated cells in amounts corresponding to the product of either 40, 80, 120 GV/cell and the total number of cells that were expected to have lysed between points x and a, and x and b. OD₆₀₀ of the cell/GV mixtures was remeasured. Observed OD₆₀₀ values from Figure 1 (points A and B), in addition to mixture OD₆₀₀ are plotted as bar charts. OD₆₀₀ values are included above each column.