About this Journal Submit a Manuscript Table of Contents
Archaea
Volume 2011 (2011), Article ID 723604, 4 pages
http://dx.doi.org/10.1155/2011/723604
Research Article

Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae

1Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann-Straße 4, Weihenstephan, 85354 Freising, Germany
2Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany

Received 1 June 2011; Accepted 21 July 2011

Academic Editor: Matthias Hess

Copyright © 2011 Angel Angelov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Picrophilus oshimae and Picrophilus torridus are free-living, moderately thermophilic and acidophilic organisms from the lineage of Euryarchaeota. With a pH optimum of growth at pH 0.7 and the ability to even withstand molar concentrations of sulphuric acid, these organisms represent the most extreme acidophiles known. So far, nothing is known about plasmid biology in these hyperacidophiles. Also, there are no genetic tools available for this genus. We have mobilized the 7.6 Kbp plasmid from P. oshimae in E. coli by introducing origin-containing transposons and described the plasmid in terms of its nucleotide sequence, copy number in the native host, mode of replication, and transcriptional start sites of the encoded ORFs. Plasmid pPO1 may encode a restriction/modification system in addition to its replication functions. The information gained from the pPO1 plasmid may prove useful in developing a cloning system for this group of extreme acidophiles.