Research Article
Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae
Table 1
Oligonucleotides used in this study.
| Primer | Sequence (5′–3′) | Description |
| RT1.for/rev | AATATGCCCTGGAGATAGCG/AGACGACACTTTCCGATACG | Used in quantitative PCR for plasmid copy number determination | RT2.for/rev | ATGCACGAATGCCTACTCTC/GGGCAAAGGAAGGCTTAAAC | 16S-Pt.for/rev | TCGTCCTTCCAGGATTACAG/CCGGCTTTGTAAATCTCCAG | RC1.rev1/2 | TCGGATTTAACAGGGCGTCTA/CCTGAACTGCCTGTTATCAAT | ORF1 inner/outer 5′RACE | RC2.rev1/2 | CAATAGGGTGGAGATGTTATAGC/TCAAAGCCCAGCCATTAGATTGC | ORF2 inner/outer 5′RACE | RC3.rev1/2 | TTACCTGACCCTTTATTTCC/TCCAGGGCGTTTAAGTAAATG | ORF3 inner/outer 5′RACE | RC4.rev1/2 | TTTCCCTGATATTCCCTTATCC/ACATCTCTTGGTATGCCTTTC | ORF4 inner/outer 5′RACE | RC5.rev1/2 | TCGGAGTAGAATTTACCTGTAG/GGATAAGGATTACCTCTGTTAG | ORF5 inner/outer 5′RACE | RC6.rev1/2 | GCATTTAGGACAGGTCGCATAC/ACTCACTACCACTTACATAC | ORF6 inner/outer 5′RACE |
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