|
| Proteins | | Genes in M. marburgensis | Genes in M. thermautotrophicus | |
| (MTBMA_c) | Number of CDS | (MTH) | |
|
| H2 activation | | | | | |
|
| MvhADG: F420-non reducing [NiFe]-hydrogenase associated with | | 15190–15160 (mvhDGAB) (MvhB is a polyferredoxin) | 4 | 1136–1133 | |
| HdrABC: heterodisulfide reductase | | 17680, 04500, 04490 | 3 | 1381, 1879, 1878 | |
| FrhABG: F420-reducing [NiFe]-hydrogenase; synthesis regulated by nickel | | 16860–16830 (frhADGB) (FrhD is an endopeptidase) | 5 | 1300–1297 | |
| Hmd: [Fe]-hydrogenase (H2-forming methylene-H4MPT dehydrogenase); synthesis regulated by nickel | | 15260 | 1 | 1142 | |
| EhaA-T: Energy-converting [NiFe]-hydrogenase; membrane-associated; Na+-dependent; mainly anabolic function | | 07840–08030 | 20 | 384–404
(EhaP twice) | |
| EhbA-Q: Energy-converting [NiFe]-hydrogenase; membrane-associated; Na+-dependent; mainly anabolic function | | 16230–16390 | 17 | 1251–1235 | |
| Nickel-responsive transcriptional regulator NikR | | 07330, 09830, 11340 | 3 | 603, 739 | |
|
| CO2 reduction to methane | | | | | |
|
| FwdA-DFGH: Tungsten-dependent formyl-MFR dehydrogenase; formed constitutively | | 01390–01450 (fwdHFGDACB) | 7 | 1553–1559 | |
| FwdA/FmdBCE: Molybdenum-dependent formyl-MFR dehydrogenase; formed only in the presence of molybdate, involving DNA-binding protein Tfx | | 13050–13070 (fmdECB) | 3 | 917–919 | |
| | 13040 | 1 | 916 | |
| Ftr: Formylmethanofuran: H4MPT formyltransferase | | 16460 | 1 | 403 | |
| Mch: Methenyl-H4MPT cyclohydrolase | | 11690 | 1 | 773 | |
| Mtd: F420-dependent methylene-H4MPT dehydrogenase; synthesis regulated by nickel | | 00500 | 1 | 1464 | |
| Mer: F420-dependent methylene-H4MPT reductase | | 03270 | 1 | 1752 | |
| MtrA-H: Methyl-H4MPT:coenzyme M methyltransferase; membrane-associated; Na+-dependent | | 15400–15470 (mtrHGFABCDE) | 7 | 1156–1163 | |
| McrABG: Methyl-coenzyme M reductase isoenzyme I; contains 5 posttranslational modifications | | 15480–15520 (mcrAGCDB) | 5 |
1164–1168 | |
| (McrCD of unknown function) | (5) |
| MrtABG: Methyl-coenzyme M reductase isoenzyme II; contains 5 posttranslational modifications | | 15120–15150 (mrtAGDB) | 4 | 1129–1132 | |
| (MrtD of unknown function) | | |
| AtwA: Mcr/Mrt-activating enzyme A2 | | 13970, 06010 | 2 | 151, 454, 1015 | |
| Putative transcriptional regulator of the mcr operon | | 05760 | 1 | 126 | |
|
| Electron transport | | | | | |
|
| 6[4Fe4S] polyferredoxin (EhaP); | |
See above | |
See above | |
| 8[4Fe4S] polyferredoxin (FwdF); | | | |
| 10[4Fe4S] polyferredoxin (EhaQ); | | | |
| 12[4Fe4S] polyferredoxin (MvhB); | | | |
| 14[4Fe4S] polyferredoxin (EhbK) | | | |
| 2[4Fe4S] ferredoxins | | 00530, 03900, 07270, 14890 | 4 | 1468, 1819, 278, 1106 | |
| 6[4Fe4S] polyferredoxin | | 08040 | 1 | 405 | |
| 8[4Fe4S] polyferredoxin | | 17360 | 1 | 1345 | |
|
| ADP phosphorylation via the Na+-motive force (generated in the MtrA-H-catalyzed reaction; see above) | | | | | |
|
| AhaA-IK: A1A0 ATP synthase complex; membrane-associated; Na+-dependent? | | 13380–13470 (ahaGDBAFCEKIH) | 10 | 952–961 | |
| NhaA: Sodium ion/proton antiporter; pH regulation | | 11540 | 1 | 760 | |
|
| Synthesis of prosthetic groups of methanogenic enzymes | | | | | |
|
| [NiFe] center of the [NiFe]-hydrogenases | | | 9 | | |
| CarAB: Carbamoyl-phosphate synthase | | 13790–13800 | | 996–998 (996+997 = CarB) | |
| HypA-F: Hydrogenase maturation factors | | 11790, 11780, 02320, 14600,06550 or 01080, 16720 | | 783, 782, 1649, 1072, 205 or 1525, 1287 | |
| FrhD and HycI: endopeptidases | | See above and 11320 | | See above and 737 | |
| FeGP cofactor of Hmd | | | 8 | | |
| HcgA-G: Hmd co-occurring proteins | | 15270–15320, 15200 (hcgABCFED,G) | | 1143–1148, 1137 | |
| HmdII and HmdIII | | 00970, 08950 | | 1512, 504 | |
| FeS-centers of hydrogenases, Fmd, Fwd, Hdr and ferredoxins | | | 3 | | |
| IscS/SufS: Cysteine desulfurase | | 17750 | | 1389 | |
| SufB/D and SufC | | 15340, 15330 | | 1150, 1149 | |
| ApbC: Putative iron-sulfur cluster transfer protein | | Not found | | 1176 | |
| Molybdopterin cofactor of formyl-MFR dehydrogenase | | | 10 | | |
| MoaABCE | | 01360, 04310, 12050, 05990 | | 1550, 1861, 809, 149 | |
| MoeAB | | 13850 or 17560, 01580 | | 1003 or 1369, 1571 | |
| MobAB | | 05930 or 09160, 01370 | | 143 or 528, 1551 | |
| B12 cofactor of methyltransferase MtrA-H | | | 4 | | |
| HemA: Glutamyl-tRNA reductase | | 13940 | | 1012 | |
| HemB: δ-Aminolevulinic acid dehydratase | | 11390 | | 744 | |
| HemC: Porphobilinogen deaminase | | 12690 | | 874 | |
| HemD: Uroporphyrinogen-III synthase | | 06170 | | 166 | |
| CysG1: Uroporphyrin-III C-methyltransferase (precorrin-2 synthesis) | | 06180 | 1 | 167 | |
| CbiX: Sirohydrochlorin cobalt chelatase | | 17830 | 14 | 1397 | |
| CbiL: Precorrin-2 C(20)-methyltransferase | | 17380 | | 1348 | |
| CbiH: Precorrin-3B C17 methylase | | 17900 | | 1403 | |
| CbiG: Cobalamin biosynthesis protein | | 17950 | | 1408 | |
| CbiF: Precorrin-4 methylase | | 09820 | | 602 | |
| CbiD: Precorrin-6A synthase | | 12040 | | 808 | |
| CbiE: Precorrin-6Y methylase | | 00990 | | 1514 | |
| CbiJ: Precorrin-6X reductase | | 13840 | | 1002 | |
| CbiT: Cobalt-precorrin-6Y C(15)-methyltransferase | | 05960 | | 146 | |
| CbiC: Precorrin-8X methylmutase | | 06760 | | 227 | |
| CbiA: Cobyrinic acid a,c-diamide synthase | | 00460 | | 1460 | |
| CobS: Cobalamin-5-phosphate synthase | | 14960 | | 1112 | |
| CbiB: Cobalamin biosynthesis protein | | 17960 | | 1409 | |
| CobN: Cobalamin biosynthesis protein | | 06860 or 09040 or 17530 | | 237 or 514 or 1363 | |
| F430 cofactor of methyl-coenzyme reductases Biosynthesis starts from uroporphyrinogen-III, probably 7 reactions are involved | | ? | (7) | ? | |
|
| Coenzyme biosynthesis | | | | | |
|
| Coenzyme F420 | | | 6 | | |
| CofA: Lactaldehyde dehydrogenase | | 13630 | | 978 | |
| CofB: L-Lactate kinase | | ? | (1) | ? | |
| CofC: 2-phospho-L-lactate guanylyltransferase | | 09930 | | 613 | |
| CofD: LPPG:FO 2-phospho-L-lactate transferase | | 14000 | | 1018 | |
| CofE: F420-0: γ-glutamyl ligase | | 14010 | | 1019 | |
| CofGH: FO synthase | | 15760,12170 | | 1198, 820 | |
| Methanofuran | | | 1 | | |
| MfnA: L-Tyrosine decarboxylase | | 15000 | | 1116 | |
| and estimated 7 unknown proteins | | ? | (7) | ? | |
| Methanopterin | | | 3 | | |
| MptA: GTP cyclohydrolase | | 15740 | | 1196 | |
| MptB: Cyclic phosphodiesterase | | 02460 | | 1668 | |
| RFAP: Ribofuranosylaminobenzene 5′-P-synthase | | 12280 | | 830 | |
| and estimated 7 unknown proteins | | ? | (7) | ? | |
| Coenzyme M | | | 5 | | |
| ComA: Phosphosulfolactate synthase | | 02530 | | 1674 | |
| ComB: 2-Phosphosulfolactate phosphatase | | 15590 | | 1182 | |
| ComC: Sulfolactate dehydrogenase | | 15830 | | 1205 | |
| ComDE: Sulfopyruvate decarboxylase | | 15840,15850 | | 1206, 1207 | |
| ComF: Sulfoacetaldehyde dehydrogenase | | ? | (1) | ? | |
| Coenzyme B | | | 4 | | |
| LeuA homolog: Isopropylmalate synthase | | 00630 or 02150 | | 1481 or 1630 | |
| LeuB homolog: Isopropylmalate dehydrogenase | | 17740 or 06370 | | 1388 or 184 | |
| LeuCD homolog: Isopropylmalate isomerase | | 02160 or 17720, 12270 or 17730 | | 1386 or 1631, 829 or 1387 | |
| and estimated 2 unknown proteins | | ? | (2) | ? | |
|
| Ion transport systems | | | | | |
|
| Ni2+ ABC transporter NikMNOQ (=CbiM1N1O1Q1) | | 02830–02860 | 4 | 1704–1707 | |
| Co2+ ABC transporter CbiM2N2O2Q2 | | 05800–05830 | 4 | 130–133 | |
| Fe2+ ABC transporter FeoAB | | 17520, 17510 | 2 | 1362, 1361 | |
| Zn2+ ABC transporter ZnuABC/ZupT | | 09840–09860, 08660 | 4 | 604–606, 473 | |
| Mg2+ transporter MgtE | | 10010 | 1 | 620 | |
| Ca2+ transporter (ATPase) | | 13830 | 1 | 1001 | |
| K+ transporter TrkAH | | 16520, 16510 | 2 | 1265, 1264 | |
| MoO42− ABC transporter ModA1B1C1 | | 13080, 13090, 13110 | 3 | 920, 921, 924 | |
| WO42− ABC transporter ModA2B2C2 | | 08720–08740 | 3 | 477–479 | |
HPO42− ABC transport system PstABCS1S2/PhoU1U2
Putative transcriptional regulator of the pst/phu operon | | 03020–03070, 03090
03000 | 8 | 1727–1732, 1734
1724 | |
|
