Acetate Activation in <i>Methanosaeta thermophila</i>: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase

<table>Activity measurement of the soluble type II pyrophosphatase (Mthe_0236). Assays contained 40 mM Tris-HCl pH 8 with 5 mM MgCl<sub>2</sub> and 1.25 <i>μ</i>g enzyme/mL. (●) activity measurement after 5 min preincubation with 1 mM MnCl<sub>2</sub>, (Δ) activity without preincubation, and (■) control without Mthe_0236. </table>

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Figure 2: Acetate Activation in <i>Methanosaeta thermophila</i>: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase 

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Acetate Activation in Methanosaeta thermophila: Characterization of the Key Enzymes Pyrophosphatase and Acetyl-CoA Synthetase

Figure 2